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Literature summary for 3.1.4.4 extracted from

  • Abousalham, A.; Teissere, M.; Gardies, A.M.; Verger, R.; Noat, G.
    Phospholipase D from soybean (Glycine max L.) suspension-cultured cells: purification, structural and enzymatic properties (1995), Plant Cell Physiol., 36, 989-996.
    View publication on PubMed
Search for more literature for 3.1.4.4

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 20-100 mM required for maximal activity in plants Brassica oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000 92000 gel filtration, SDS-PAGE Brassica oleracea
90000 92000 calculated from amino acid analysis Brassica oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipid + H2O Brassica oleracea phosphoric ester hydrolysis phosphatidic acid + alcohol
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
 savoy cabbage
-

Purification (Commentary)

Purification (Comment) Organism
complete to homogeneity Brassica oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Brassica oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1492
-
-
 Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O
-
 Brassica oleracea 1,2-diacylglycerophosphate + choline
-
 ?
phospholipid + H2O phosphoric ester hydrolysis Brassica oleracea phosphatidic acid + alcohol
-
 ?

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 Ca2+ dependent Brassica oleracea