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Literature summary for 3.1.4.4 extracted from

  • Tzur, R.; Shapiro, B.
    Purification of phospholipase D from peanuts (1972), Biochim. Biophys. Acta, 280, 290-296.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ethyl ether
-
Arachis hypogaea
phosphatidic acid only with addition of lecithin and SDS Arachis hypogaea
SDS
-
Arachis hypogaea

Inhibitors

Inhibitors Comment Organism Structure
albumin
-
Arachis hypogaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
phosphatidylcholine
-
Arachis hypogaea
2.5
-
phosphatidylcholine transphosphaditylation in presence of methanol Arachis hypogaea

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 20-100 mM required for maximal activity in plants Arachis hypogaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipid + alcohol Arachis hypogaea transphosphaditylation phospholipid + alcohol
-
?
phospholipid + H2O Arachis hypogaea phosphoric ester hydrolysis phosphatidic acid + alcohol
-
?

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
peanut
-

Purification (Commentary)

Purification (Comment) Organism
partial Arachis hypogaea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
234
-
-
Arachis hypogaea

Storage Stability

Storage Stability Organism
-20°C, lyophilyzed, 2 month, no loss of activity Arachis hypogaea
4°C, neutral pH, 50 days, no loss of activity Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + ethanolamine
-
Arachis hypogaea phosphatidylethanolamine + choline
-
?
phosphatidylcholine + H2O
-
Arachis hypogaea 1,2-diacylglycerophosphate + choline
-
?
phosphatidylcholine + methanol
-
Arachis hypogaea phosphatidylmethanol + choline
-
?
phospholipid + alcohol transphosphaditylation Arachis hypogaea phospholipid + alcohol
-
?
phospholipid + H2O phosphoric ester hydrolysis Arachis hypogaea phosphatidic acid + alcohol
-
?