General Stability | Organism |
---|---|
enzyme dilutions retain activity at neutral pH | Arachis hypogaea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 20-100 mM required for maximal activity in plants | Arachis hypogaea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
sedimentation | Arachis hypogaea |
46000 | 50000 | gel filtration in the presence of reducing agents, SDS-PAGE | Arachis hypogaea |
50000 | - |
? * 50000, ? * 22000 time-dependent dissociation into smaller subunits possible, gel filtration in the presence of reducing agents | Arachis hypogaea |
200000 | - |
time-dependent dissociation into smaller subunits possible, gel filtration, SDS-PAGE | Arachis hypogaea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phospholipid + H2O | Arachis hypogaea | phosphoric ester hydrolysis | phosphatidic acid + alcohol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arachis hypogaea | - |
peanut | - |
Purification (Comment) | Organism |
---|---|
complete to homogeneity | Arachis hypogaea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Arachis hypogaea | - |
Storage Stability | Organism |
---|---|
-20°C, neutral pH, 0.5 M glycerol, 20 days, no loss of activity | Arachis hypogaea |
-20°C, neutral pH, few days, 95% loss of activity | Arachis hypogaea |
20°C, neutral pH, 15 days, no loss of activity | Arachis hypogaea |
4°C, neutral pH, 50 days, no loss of activity | Arachis hypogaea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phospholipid + H2O | phosphoric ester hydrolysis | Arachis hypogaea | phosphatidic acid + alcohol | - |
? |
Subunits | Comment | Organism |
---|---|---|
polymer | ? * 50000, ? * 22000 time-dependent dissociation into smaller subunits possible, gel filtration in the presence of reducing agents | Arachis hypogaea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
- |
Arachis hypogaea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
irreversible loss of activity at acid pH | Arachis hypogaea |