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Literature summary for 3.1.4.4 extracted from

  • Heller, M.; Mozes, N.; Peri, I.; Maes, E.
    Phospholipase D from peanut seeds. Final purification and some properties of the enzyme (1974), Biochim. Biophys. Acta, 369, 397-410.
No PubMed abstract available

General Stability

General Stability Organism
enzyme dilutions retain activity at neutral pH Arachis hypogaea

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 20-100 mM required for maximal activity in plants Arachis hypogaea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
sedimentation Arachis hypogaea
46000 50000 gel filtration in the presence of reducing agents, SDS-PAGE Arachis hypogaea
50000
-
? * 50000, ? * 22000 time-dependent dissociation into smaller subunits possible, gel filtration in the presence of reducing agents Arachis hypogaea
200000
-
time-dependent dissociation into smaller subunits possible, gel filtration, SDS-PAGE Arachis hypogaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipid + H2O Arachis hypogaea phosphoric ester hydrolysis phosphatidic acid + alcohol
-
?

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
peanut
-

Purification (Commentary)

Purification (Comment) Organism
complete to homogeneity Arachis hypogaea

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Arachis hypogaea
-

Storage Stability

Storage Stability Organism
-20°C, neutral pH, 0.5 M glycerol, 20 days, no loss of activity Arachis hypogaea
-20°C, neutral pH, few days, 95% loss of activity Arachis hypogaea
20°C, neutral pH, 15 days, no loss of activity Arachis hypogaea
4°C, neutral pH, 50 days, no loss of activity Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phospholipid + H2O phosphoric ester hydrolysis Arachis hypogaea phosphatidic acid + alcohol
-
?

Subunits

Subunits Comment Organism
polymer ? * 50000, ? * 22000 time-dependent dissociation into smaller subunits possible, gel filtration in the presence of reducing agents Arachis hypogaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
Arachis hypogaea

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
irreversible loss of activity at acid pH Arachis hypogaea