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Literature summary for 3.1.4.4 extracted from
- Phospholipase D from savoy cabbage: purification and preliminary kinetic characterization (1979), Biochemistry, 18, 5348-5353.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Brassica oleracea |  |
General Stability
| General Stability | Organism |
|---|---|
| 50% ethylene glycol maintain stability of purified enzyme | Brassica oleracea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.64 | - |
dihexanoylphosphatidylcholine | - |
Brassica oleracea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Brassica oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 112500 | 116600 | gel filtration, SDS-PAGE | Brassica oleracea |
| 112500 | 116600 | sedimentation | Brassica oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospholipid + H2O | Brassica oleracea | phosphoric ester hydrolysis | phosphatidic acid + alcohol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica oleracea | - |
savoy cabbage | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| complete to homogeneity | Brassica oleracea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
- |
Brassica oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, Tes, 10% inositol,1 month, no loss of activity | Brassica oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihexanoylphosphatidylcholine + H2O | - |
Brassica oleracea | dihexanoylglycerophosphate + choline | - |
? | |
| phosphatidylcholine + H2O | - |
Brassica oleracea | 1,2-diacylglycerophosphate + choline | - |
? | |
| phospholipid + H2O | phosphoric ester hydrolysis | Brassica oleracea | phosphatidic acid + alcohol | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | Ca2+ dependent | Brassica oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | Ca2+ dependent | Brassica oleracea |
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