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Literature summary for 3.1.4.39 extracted from

  • Borchani, L.; Sassi, A.; Ben Gharsa, H.; Safra, I.; Shahbazzadeh, D.; Ben Lasfar, Z.; El Ayeb, M.
    The pathological effects of heminecrolysin, a dermonecrotic toxin from Hemiscorpius lepturus scorpion venom are mediated through its lysophospholipase D activity (2013), Toxicon, 68, 30-39.
    View publication on PubMed
Search for more literature for 3.1.4.39

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenantroline 50% inhibition at 2.5 mM, 70% inhibition Hemiscorpius lepturus
EDTA 98% inhibition Hemiscorpius lepturus
EGTA 48% inhibition Hemiscorpius lepturus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.097
-
 1-alkyl-sn-glycero-3-phosphocholine pH 7.4, 37°C Hemiscorpius lepturus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Hemiscorpius lepturus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, dependent on Hemiscorpius lepturus
Mg2+ activates, dependent on Hemiscorpius lepturus
Zn2+ activates Hemiscorpius lepturus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000 Hemiscorpius lepturus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-alkyl-sn-glycero-3-phosphocholine + H2O Hemiscorpius lepturus
-
 1-alkyl-sn-glycerol 3-phosphate + choline
-
 ?
additional information Hemiscorpius lepturus the enzyme is active on human macrophages, erythrocytes, Jurkat and HeLa cells, and on chicken embryo chorioallantoic membrane ?
-
 ?

Organism

Organism UniProt Comment Textmining
Hemiscorpius lepturus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Hemiscorpius lepturus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-alkyl-sn-glycero-3-phosphocholine + H2O
-
 Hemiscorpius lepturus 1-alkyl-sn-glycerol 3-phosphate + choline
-
 ?
additional information the enzyme is active on human macrophages, erythrocytes, Jurkat and HeLa cells, and on chicken embryo chorioallantoic membrane Hemiscorpius lepturus ?
-
 ?
additional information the enzyme shows slight activity with sphingomyelin, but no activity with phosphatidylcholine Hemiscorpius lepturus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 33000 Hemiscorpius lepturus

Synonyms

Synonyms Comment Organism
Heminecrolysin
-
 Hemiscorpius lepturus
lysophospholipase D
-
 Hemiscorpius lepturus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Hemiscorpius lepturus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Hemiscorpius lepturus

General Information

General Information Comment Organism
additional information Heminecrolysin does not affect glycophorin A expression Hemiscorpius lepturus
physiological function Heminecrolysin induces erythrocyte hemolysis via lysophospholipase D activity. Heminecrolysin efficiently binds to erythrocyte's membrane and provokes phosphatidylserine translocation without cleavage of glycophorin A, suggesting that, unlike spider's phospholipase D, complement is activated only via the classical pathway. Heminecrolysin induces pro-inflammatory cytokine release and causes necrosis in the vascular tissue Hemiscorpius lepturus