Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, ATX alone and in complex with a small-molecule inhibitor HA155, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement and single-wavelength anomalous dispersion | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
A218V | site-directed mutagenesis, a hydrophobic binding pocket mutant, the mutant shows reduced lysoPLD activity levels compared to the wild-type | Rattus norvegicus |
A305E | site-directed mutagenesis, a hydrophobic binding pocket mutant, the mutant shows reduced lysoPLD activity levels compared to the wild-type | Rattus norvegicus |
F211A | site-directed mutagenesis, a hydrophobic binding pocket mutant, the mutant shows reduced lysoPLD activity levels compared to the wild-type | Rattus norvegicus |
F274E | site-directed mutagenesis, a hydrophobic binding pocket mutant, the mutant shows reduced lysoPLD activity levels compared to the wild-type | Rattus norvegicus |
F275Q | site-directed mutagenesis, a hydrophobic binding pocket mutant, the mutant shows reduced lysoPLD activity levels compared to the wild-type | Rattus norvegicus |
L214H | site-directed mutagenesis, a hydrophobic binding pocket mutant, the mutant shows reduced lysoPLD activity levels compared to the wild-type | Rattus norvegicus |
S170E | site-directed mutagenesis, a hydrophobic binding pocket mutant, the mutant shows reduced lysoPLD activity levels compared to the wild-type | Rattus norvegicus |
Y307Q | site-directed mutagenesis, a hydrophobic binding pocket mutant, the mutant shows reduced lysoPLD activity levels compared to the wild-type | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HA155 | the boron atom on one end of the inhibitor forms a reversible covalent bond with the nucleophile hydroxyl group of Thr209. One of the two boron hydroxyl groups is further stabilized between the two zinc ions, binding structure, overview | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, active site bound | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
about | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | binding of ATX to activated platelets and lymphocytes in an integrin-dependent manner mediated by the consecutive cysteine-rich somatomedin-B-like, SMB, domains | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q64610 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | binding of ATX to activated platelets and lymphocytes in an integrin-dependent manner mediated by the consecutive cysteine-rich somatomedin-B-like, SMB, domains | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | ATX has several distinct domains. At the N-terminus it harbours two consecutive cysteine-rich somatomedin-B-like, SMB, domains, a fold known to be involved in protein-protein interactions. The central catalytic phosphodiesterase, PDE, domain follows the SMB domains. At its C-terminus, ATX contains a nuclease-like, NUC, domain, which is catalytically inert and might be involved in substrate binding and presentation | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
ATX | - |
Rattus norvegicus |
autotaxin | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
additional information | structural basis of substrate discrimination and integrin binding by autotaxin, ATX, that interacts with cell-surface integrins via its N-terminal somatomedin-B-like domains, using an atypical mechanism, overview | Rattus norvegicus |
physiological function | ATX promotes localized LPA signaling | Rattus norvegicus |