Cloned (Comment) | Organism |
---|---|
co-transfection of MBEC4 cells with 2 plasmids, mlysoPLDpCAGGS26 and pSV2-neo, screening by Western blotting and immunocytochemistry with an anti-ATX mAb (4F1)14 and by measuring the lysoPLD activities of the culture media | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
approx., Western blot, ATX expressed in MBEC4-ATX cells | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lysophosphatidylcholine + H2O | Mus musculus | - |
lysophosphatidic acid + choline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood plasma | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
approx. 400 micromol/min/ml, mice are treated with control rat IgG, plasma samples (10 microl) incubate with 4 mmol/l lysophosphatidylcholine, activity determined by enzymatic photometric method using choline oxidase at 560 nm, 37°C | Mus musculus |
additional information | - |
approx. 42 micromol/min/ml, mice are treated with anti-ATX mAb, plasma samples (10 microl) incubate with 4 mmol/l lysophosphatidylcholine, activity determined by enzymatic photometric method using choline oxidase at 560 nm, 37°C | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lysophosphatidylcholine + H2O | - |
Mus musculus | lysophosphatidic acid + choline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATX | - |
Mus musculus |
autotaxin | - |
Mus musculus |
lysoPLD | - |
Mus musculus |