Literature summary for 3.1.4.39 extracted from

Liu, X.W.; Sok, D.E.; Yook, H.S.; Sohn, C.B.; Chung, Y.J.; Kim, M.R.
Inhibition of lysophospholipase D activity by unsaturated lysophosphatidic acids or seed extracts containing 1-linoleoyl and 1-oleoyl lysophosphatidic acid (2007), J. Agric. Food Chem., 55, 8717-8722.

Search for more literature for 3.1.4.39

Inhibitors

Inhibitors	Comment	Organism	Structure
1-arachidonoyl-lysophosphatidic acid	Clarification of the structural importance of acyl chain for the inhibitory effect of lysophosphatidic acid on lysoPLD activity. Lysophosphatidic acids with polyunsaturated acyl chains are more potent than those with saturated acyl chains in the inhibition of serum lysoPLD activity	Bos taurus
1-arachidoyl-lysophosphatidic acid	Clarification of the structural importance of acyl chain for the inhibitory effect of lysophosphatidic acid on lysoPLD activity. Lysophosphatidic acids with polyunsaturated acyl chains are more potent than those with saturated acyl chains in the inhibition of serum lysoPLD activity	Bos taurus
1-hexanoyl-lysophosphatidic acid	Clarification of the structural importance of acyl chain for the inhibitory effect of lysophosphatidic acid on lysoPLD activity. Lysophosphatidic acids with polyunsaturated acyl chains are more potent than those with saturated acyl chains in the inhibition of serum lysoPLD activity	Bos taurus
1-linoleoyl-lysophosphatidic acid	Clarification of the structural importance of acyl chain for the inhibitory effect of lysophosphatidic acid on lysoPLD activity. Lysophosphatidic acids with polyunsaturated acyl chains are more potent than those with saturated acyl chains in the inhibition of serum lysoPLD activity	Bos taurus
1-myristoyl-lysophosphatidic acid	competitive inhibition of lysoPLD. Clarification of the structural importance of acyl chain for the inhibitory effect of lysophosphatidic acid on lysoPLD activity. Lysophosphatidic acids with polyunsaturated acyl chains are more potent than those with saturated acyl chains in the inhibition of serum lysoPLD activity	Bos taurus
1-oleoyl-lysophosphatidic acid	Clarification of the structural importance of acyl chain for the inhibitory effect of lysophosphatidic acid on lysoPLD activity. Lysophosphatidic acids with polyunsaturated acyl chains are more potent than those with saturated acyl chains in the inhibition of serum lysoPLD activity	Bos taurus
1-palmitoyl-lysophosphatidic acid	Clarification of the structural importance of acyl chain for the inhibitory effect of lysophosphatidic acid on lysoPLD activity. Lysophosphatidic acids with polyunsaturated acyl chains are more potent than those with saturated acyl chains in the inhibition of serum lysoPLD activity	Bos taurus
1-stearoyl-lysophosphatidic acid	Clarification of the structural importance of acyl chain for the inhibitory effect of lysophosphatidic acid on lysoPLD activity. Lysophosphatidic acids with polyunsaturated acyl chains are more potent than those with saturated acyl chains in the inhibition of serum lysoPLD activity	Bos taurus
additional information	several extracts from edible plant seeds (sesame, sunflower, soybean) tested for the inhibition of lysoPLD activity, show a inhibition (>80%) of lysoPLD, fluorometric assay is favored over the spectrophotometric assay, ESI-MS/MS spectrum and MRM spectrum of major lysophosphatidic acids from seed lipid	Bos taurus
sphingosine-1-phosphate	Clarification of the structural importance of acyl chain for the inhibitory effect of lysophosphatidic acid on lysoPLD activity. Lysophosphatidic acids with polyunsaturated acyl chains are more potent than those with saturated acyl chains in the inhibition of serum lysoPLD activity; competitive inhibition of lysoPLD	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
from fetal bovine serum. SDS-PAGE, protein detection by silver staining	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
blood serum	-	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	Spectrophotometric assay of LysoPLD activity. Determination of lysoPLD activity by measuring the amount of choline released from exogenously added LPC. Fluorometric assay for LysoPLD activity. Determination of LysoPLD activity using a fluorogenic substrate, [5-[[(23S)-33-(4-[(E)-[4-(dimethylamino)phenyl]diazenyl]phenyl)-20,23-dihydroxy-20-oxido-15,26,33-trioxo-3,6,9,12,19,21,25-heptaoxa-16,32-diaza-20-phosphatritriacont-1-yl]carbamoyl]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)phenyl]acetic acid (FS-3)	Bos taurus

Synonyms

Synonyms	Comment	Organism
lysophospholipase D	-	Bos taurus
lysoPLD	-	Bos taurus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00021	-	1-linoleoyl-lysophosphatidic acid	most potent in the inhibition of lysoPLD activity, based on fluoresecent assay, same order of inhibitory potency among lysophosphatidic acid analogs with different acyl chains in the spectrophotometric assay	Bos taurus
0.00021	-	sphingosine-1-phosphate	SIP, inhibition of lysoPLD activity, fluorometric assay	Bos taurus
0.00055	-	1-arachidonoyl-lysophosphatidic acid	inhibition of lysoPLD activity, based on fluoresecent assay, same order of inhibitory potency among lysophosphatidic acid analogs with different acyl chains in the spectrophotometric assay	Bos taurus
0.0012	-	1-oleoyl-lysophosphatidic acid	inhibition of lysoPLD activity, based on fluoresecent assay, same order of inhibitory potency among lysophosphatidic acid analogs with different acyl chains in the spectrophotometric assay	Bos taurus
0.00141	-	1-palmitoyl-lysophosphatidic acid	inhibition of lysoPLD activity,size of acyl chain is crucial for the maximal inhibitory action, fluorometric assay	Bos taurus
0.0018	-	1-linoleoyl-lysophosphatidic acid	competitive inhibition, spectrophotometric assay	Bos taurus
0.0018	-	sphingosine-1-phosphate	SIP, inhibition of lysoPLD activity, spectrophotometric assay	Bos taurus
0.00209	-	1-stearoyl-lysophosphatidic acid	inhibition of lysoPLD activity,size of acyl chain is crucial for the maximal inhibitory action, fluorometric assay	Bos taurus
0.0034	-	1-arachidonoyl-lysophosphatidic acid	inhibition of lysoPLD activity, spectrophotometric assay	Bos taurus
0.005	-	1-myristoyl-lysophosphatidic acid	most potent in inhibiting lysoPLD activity, spectrophotometric assay	Bos taurus
0.0065	-	1-myristoyl-lysophosphatidic acid	most potent in inhibiting lysoPLD activity, fluorometric assay	Bos taurus
0.0096	-	1-oleoyl-lysophosphatidic acid	inhibition of lysoPLD activity, spectrophotometric assay	Bos taurus
0.01031	-	1-arachidoyl-lysophosphatidic acid	inhibition of lysoPLD activity, fluorometric assay	Bos taurus
0.0104	-	1-palmitoyl-lysophosphatidic acid	inhibition of lysoPLD activity,size of acyl chain is crucial for the maximal inhibitory action, spectrophotometric assay	Bos taurus
0.01299	-	1-hexanoyl-lysophosphatidic acid	inhibition of lysoPLD activity, fluorometric assay	Bos taurus
0.0258	-	1-stearoyl-lysophosphatidic acid	inhibition of lysoPLD activity,size of acyl chain is crucial for the maximal inhibitory action, spectrophotometric assay	Bos taurus
0.06	-	1-arachidoyl-lysophosphatidic acid	> 60 microM, inhibition of lysoPLD activity, spectrophotometric assay	Bos taurus
0.06	-	1-hexanoyl-lysophosphatidic acid	inhibition of lysoPLD activity, > 60 microM, size of acyl chain is crucial for the maximal inhibitory action, spectrophotometric assay	Bos taurus

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Release 2023.1 (January 2023)