Literature summary for 3.1.4.39 extracted from

Pradere, J.P.; Tarnus, E.; Gres, S.; Valet, P.; Saulnier-Blache, J.S.
Secretion and lysophospholipase D activity of autotaxin by adipocytes are controlled by N-glycosylation and signal peptidase (2007), Biochim. Biophys. Acta, 1771, 93-102.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in COS-7 cells	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
DELTAA30-G41	deletion mutants are generated from plasmid pcDNA-mATX, encompasses furin site	Mus musculus
DELTAA30-I33	deletion mutants are generated from plasmid pcDNA-mATX, between signal peptidase and furin site	Mus musculus
DELTAA36-E40	deletion mutants are generated from plasmid pcDNA-mATX	Mus musculus
DELTAC25	deletion mutants are generated from plasmid pcDNA-mATX, amino acid -3 referring to the potential signal peptidase cleavage site	Mus musculus
DELTAE40-P43	deletion mutants are generated from plasmid pcDNA-mATX	Mus musculus
DELTAG27	deletion mutants are generated from plasmid pcDNA-mATX, amino acid -1 referring to the potential signal peptidase cleavage site	Mus musculus
DELTAG27-A30	deletion mutants are generated from plasmid pcDNA-mATX, contain potential signal peptidase clevage site	Mus musculus
DELTAG27-R35	deletion mutants are generated from plasmid pcDNA-mATX, encompasses both furin and signal peptidase cleavage sites	Mus musculus
DELTAL46-S49	deletion mutants are generated from plasmid pcDNA-mATX	Mus musculus
DELTAN23	deletion mutants are generated from plasmid pcDNA-mATX, amino acid -5 referring to the potential signal peptidase cleavage site	Mus musculus
DELTAN23-G27	deletion mutants are generated from plasmid pcDNA-mATX, encompasses -1 and -3 amino acids referring to the potential signal peptidase clevage site	Mus musculus
DELTAN410	deletion mutants are generated from plasmid pcDNA-mATX, contains a N-glycosylation site, point deletion of the amino-acid N410 inhibits lysophospholipase D activity of ATX, does not modify the ATX secretion and strongly inhibits ATX activity	Mus musculus
DELTAN53	deletion mutants are generated from plasmid pcDNA-mATX, contains a N-glycosylation site, does not modify the ATX secretion and slightly reduces (25%) ATX activity	Mus musculus
DELTAN53/DELTAN410	deletion mutants are generated from plasmid pcDNA-mATX, two N-glycosylation sites, double point deletion of the amino-acids N53 and N410 inhibits secretion of ATX, without altering the ATX amount in cell homogenate	Mus musculus
DELTAP43-L46	deletion mutants are generated from plasmid pcDNA-mATX	Mus musculus
DELTAR32-R35	deletion mutants are generated from plasmid pcDNA-mATX, furine site	Mus musculus
DELTAS49-N53	deletion mutants are generated from plasmid pcDNA-mATX, contains N-glycosylation site	Mus musculus
DELTAV12-G27	deletion mutants are generated from plasmid pcDNA-mATX, hydrophobic domain of signal peptide and signal peptide cleavage site, ATX secretion is suppressed	Mus musculus
DELTAV12-V22	deletion mutants are generated from plasmid pcDNA-mATX, hydrophobic domain of signal peptide, ATX secretion is suppressed	Mus musculus
additional information	deletions of the amino acids N53 and N410 lead to a reduction of the molecular weight of ATX	Mus musculus

Inhibitors

Inhibitors	Comment	Organism
brefeldin-A	inhibitor of trans-Golgi transport, inhibits secretion of ATX	Mus musculus
Globomycin	Treatment with the signal peptidase inhibitor inhibits ATX secretion by adipocytes treated for 6 h	Mus musculus
lactacystin	proteasome inhibitor, restores the detection of ATX in cell homogenate of the mutants DELTAV12-V22 and DELTAV12-G27, Synthesis and secretion of ATX are highly dependent on the hydrophobic core of the signal peptide, but not on the amino acid composition the putative signal peptidase cleavage site	Mus musculus
additional information	possible involement of furin in secretion of ATX by adipocytes tested, furin inhibitor decanoyl-ArgValLysArgchloromethylketone does not modify secretion or lysophospholipase D activity of ATX	Mus musculus
N-glycosidase	treatment with N-glycosidase inhibits lysophospholipase D activity of ATX. N-glycosylation of ATX strongly influences its secretion and its lysoPLD activity	Mus musculus
tunicamycin	inhibits secretion of ATX	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	results suggest that cell ATX behaves mainly as a soluble protein	Mus musculus	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
120000	-	analyzed by Western blot, ATX is undetectable in conditioned media from undifferentiated 3T3F442A preadipocytes. Conditioned media treated with N-glycosidase and O-glycosidase, N-glycosidase leads to a reduction of 9.6 kDa apparent molecular weight of ATX, O-glycosidase is without influence	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
lysophosphatidylcholine + H2O	Mus musculus	-	lysophosphatidic acid + choline	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Mus musculus

Purification (Commentary)

Purification (Comment)	Organism
Western blot in cell homogenates requires a pre-purification with concanavalin A-sepharose	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
adipocyte	Immunocyto-fluorescence microscopy reveals that ATX is detected in 3T3F442A adipocytes and is almost undetectable in 3T3F442A preadipocytes. 3T3F442A mouse preadipose cell line	Mus musculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	lysophospholipase D activity is measured by conversion of radiolabeled lysophosphatidylcholine into radiolabeled lysophosphatidic acid	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
lysophosphatidylcholine + H2O	-	Mus musculus	lysophosphatidic acid + choline	-	?

Synonyms

Synonyms	Comment	Organism
ATX	-	Mus musculus
autotaxin	-	Mus musculus
ectonucleotide pyrophosphatase phosphodiesterase-2	-	Mus musculus
ENPP2	-	Mus musculus
lysophospholipase D	-	Mus musculus