Literature summary for 3.1.4.39 extracted from

Morishige, J.; Touchika, K.; Tanaka, T.; Satouchi, K.; Fukuzawa, K.; Tokumura, A.
Production of bioactive lysophosphatidic acid by lysophospholipase D in hen egg white (2007), Biochim. Biophys. Acta, 1771, 491-499.

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Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	inhibitory effects of 1,10-phenanthroline at different concentrations on lysoPLD activity against palmitoyl-lysophosphatidylcholine	Gallus gallus
bovine serum albumin	concentration-dependent inhibition on lysoPLD activity in egg white during 24 h incubation	Gallus gallus
EDTA	inhibitory effects of EDTA at different concentrations on lysoPLD activity against palmitoyl-lysophosphatidylcholine. Inhibitory effect disappears when Mn2+ or Co2+ are added with EDTA to the egg white. Co-addition of Zn2+, Ni2+, or Cu2+ with EDTA results in 2-3times higher lysoPLD activity	Gallus gallus
lysozyme	concentration-dependent inhibition on lysoPLD activity in egg white during 24 h incubation	Gallus gallus
additional information	Hypothesis that a protein in hen egg white masks the lysoPLD activity in undiluted egg white, and that dilution at more than 10times results in a high decline of the inhibitory activity	Gallus gallus
palmitoyl-lysophosphatidic acid	concentration-dependent inhibition of lysoPLD activity in egg white during 24 h incubation. More than 100 microM of lysophosphatidic acid needed to inhibit significantly lysoPLD activity of hen egg white	Gallus gallus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Gallus gallus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	lysoPLD activity is increased when Co2+ is added. Addition of Zn2+, Mn2+, Ni2+, or Co2+ to diluted egg white alters preference patterns of lysoPLD toward choline-containing substrates	Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
110000	-	Western blot analysis of AXT/lysoPLD	Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
lysophosphatidylcholine + H2O	Gallus gallus	-	lysophosphatidic acid + choline	-	?

Organism

Organism	UniProt	Comment	Textmining
Gallus gallus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
egg white	lysoPLD secreted in several animal body fluids. Hen egg white originates mainly from the hen oviduct fluid, which contains various bioactive molecules acting on the oviduct, ova, and sperm	Gallus gallus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	lysoPLD activity of diluted or undiluted egg white measured based on enzyme-linked fluorometric measurement of choline produced together with lysophosphatidic acid from exogenous lysophosphatidylcholine. LysoPLD activity measured by determining lysophosphatidic acid and choline by MALDI-TOF-MS, mass spectrometric and enzyme-linked fluorometric analysis. Quantitative analysis of lysophosphatidic acid by MALDI-TOF-MS using a phosphate-capture molecule. Production of lysophosphatidic acid during incubation of hen egg white, changes in the levels of fatty acyl groups	Gallus gallus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
lysophosphatidylcholine + H2O	-	Gallus gallus	lysophosphatidic acid + choline	-	?

Synonyms

Synonyms	Comment	Organism
lysophospholipase D	-	Gallus gallus
lysoPLD	-	Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Gallus gallus

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Release 2023.1 (January 2023)