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Literature summary for 3.1.4.35 extracted from

  • Day, J.P.; Cleghon, V.; Houslay, M.D.; Davies, S.A.
    Regulation of a Drosophila melanogaster cGMP-specific phosphodiesterase by prenylation and interaction with a prenyl-binding protein (2008), Biochem. J., 414, 363-374.
    View publication on PubMed
Search for more literature for 3.1.4.35

Protein Variants

Protein Variants Comment Organism
C1128S the unprenylated mutant enzyme is mainly localized to the cytosol Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
sildenafil
-
 Drosophila melanogaster
zaprinast
-
 Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0281
-
 3',5'-cAMP wild type enzyme, in 50 mM KCl, 10 mM EGTA, 1.92 mM MgCl2, 1 mM dithiothreitol, and 50 mM HEPES, pH 7.4 Drosophila melanogaster
0.0354
-
 3',5'-cAMP mutant enzyme C1128S, in 50 mM KCl, 10 mM EGTA, 1.92 mM MgCl2, 1 mM dithiothreitol, and 50 mM HEPES, pH 7.4 Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol C1128S-PDE5/6 mutant form is located primarily in the cytosol, although some residual association occurs at the apical membrane Drosophila melanogaster 5829
-
plasma membrane PDE5/6 is exclusively associated with the apical membrane Drosophila melanogaster 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 SDS-PAGE Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Drosophila melanogaster prenylation of the enzyme is required for plasma-membrane targeting ?
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
S2 cell
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-cAMP + H2O
-
 Drosophila melanogaster 5'-AMP
-
 ?
3',5'-cGMP + H2O
-
 Drosophila melanogaster 5'-GMP
-
 ?
additional information prenylation of the enzyme is required for plasma-membrane targeting Drosophila melanogaster ?
-
 ?

Synonyms

Synonyms Comment Organism
cGMP-PDE
-
 Drosophila melanogaster
cGMP-specific phosphodiesterase
-
 Drosophila melanogaster
PDE5/6
-
 Drosophila melanogaster