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Literature summary for 3.1.4.2 extracted from

  • Larson, T.J.; van Loo-Bhattacharya, A.T.
    Purification and characterization of glpQ-encoded glycerophosphodiester phosphodiesterase from Escherichia coli K-12 (1988), Arch. Biochem. Biophys., 260, 577-584.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 1 mM Escherichia coli
EGTA complete inhibition at 1 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
glycerophosphoethanolamine
-
Escherichia coli
0.28
-
glycerophosphocholine addition of Ca2+ decreases Km and increases Vmax. Escherichia coli
0.35
-
glycerophosphoglycerol
-
Escherichia coli
0.59
-
glycerophosphoserine
-
Escherichia coli
1
-
glycerophosphoinositol
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ fully restored activity if chelating agents used Escherichia coli
Cd2+ fully restored activity if chelating agents used Escherichia coli
Cu2+ partially restored activity if chelating agents used Escherichia coli
Mn2+ partially restored activity if chelating agents used Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
SDS-PAGE analysis Escherichia coli
70000
-
gel filtration chromatography Escherichia coli
75000
-
native PAGE analysis Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogenity Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
325
-
90% homogenity Escherichia coli

Storage Stability

Storage Stability Organism
-75°C, 25 mM Tris-HCl pH 7.4, 0.1 mM DTT, 10% glycerol, several months Escherichia coli
room temperature, 0.02 M Tris-HCl pH 7.5, 24 h Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerophosphoethanolamine + H2O
-
Escherichia coli ?
-
?
glycerophosphoglycerol + H2O
-
Escherichia coli glycerol + glycerol 3-phosphate
-
?
glycerophosphoinositol + H2O
-
Escherichia coli inositol + glycerol 3-phosphate
-
?
glycerophosphoserine + H2O
-
Escherichia coli serine + glycerol 3-phosphate
-
?
sn-glycero 3-phosphorylcholine + H2O
-
Escherichia coli sn-glycerol 3-phosphate + choline
-
?
sn-glycero 3-phosphorylethanolamine + H2O
-
Escherichia coli sn-glycerol 3-phosphate + ethanolamine
-
?

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 37500-40000, native PAGE and SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
glycerophosphodiester phosphodiesterase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
220
-
sn-glycero 3-phosphorylcholine
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
using two-step assay Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.7 9 less than 50% activity below pH6 6.7 and above pH 9.0 Escherichia coli