Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition at 1 mM | Escherichia coli | |
EGTA | complete inhibition at 1 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
glycerophosphoethanolamine | - |
Escherichia coli | |
0.28 | - |
glycerophosphocholine | addition of Ca2+ decreases Km and increases Vmax. | Escherichia coli | |
0.35 | - |
glycerophosphoglycerol | - |
Escherichia coli | |
0.59 | - |
glycerophosphoserine | - |
Escherichia coli | |
1 | - |
glycerophosphoinositol | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | fully restored activity if chelating agents used | Escherichia coli | |
Cd2+ | fully restored activity if chelating agents used | Escherichia coli | |
Cu2+ | partially restored activity if chelating agents used | Escherichia coli | |
Mn2+ | partially restored activity if chelating agents used | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE analysis | Escherichia coli |
70000 | - |
gel filtration chromatography | Escherichia coli |
75000 | - |
native PAGE analysis | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogenity | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
325 | - |
90% homogenity | Escherichia coli |
Storage Stability | Organism |
---|---|
-75°C, 25 mM Tris-HCl pH 7.4, 0.1 mM DTT, 10% glycerol, several months | Escherichia coli |
room temperature, 0.02 M Tris-HCl pH 7.5, 24 h | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerophosphoethanolamine + H2O | - |
Escherichia coli | ? | - |
? | |
glycerophosphoglycerol + H2O | - |
Escherichia coli | glycerol + glycerol 3-phosphate | - |
? | |
glycerophosphoinositol + H2O | - |
Escherichia coli | inositol + glycerol 3-phosphate | - |
? | |
glycerophosphoserine + H2O | - |
Escherichia coli | serine + glycerol 3-phosphate | - |
? | |
sn-glycero 3-phosphorylcholine + H2O | - |
Escherichia coli | sn-glycerol 3-phosphate + choline | - |
? | |
sn-glycero 3-phosphorylethanolamine + H2O | - |
Escherichia coli | sn-glycerol 3-phosphate + ethanolamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, 2 * 37500-40000, native PAGE and SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
glycerophosphodiester phosphodiesterase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
220 | - |
sn-glycero 3-phosphorylcholine | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
using two-step assay | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 9 | less than 50% activity below pH6 6.7 and above pH 9.0 | Escherichia coli |