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Literature summary for 3.1.4.2 extracted from
- A novel glycerophosphodiester phosphodiesterase improves phosphate deficiency tolerance in rice (2018), Plant Cell Environ., 42, 1167-1179 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Oryza sativa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.56 | - |
sn-glycero-3-phosphocholine | at pH 7.5 and 45°C | Oryza sativa | |
| 1.06 | - |
sn-glycero-3-phosphoethanolamine | at pH 7.5 and 45°C | Oryza sativa | |
| 7.25 | - |
sn-glycero-3-phosphoinositol | at pH 7.5 and 45°C | Oryza sativa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | the enzyme shows maximum activity at very low concentrations (less than 0.05 mM) of Co2+ | Oryza sativa | |
| Mg2+ | preferred cofactor. The enzyme activity increases with increasing concentrations of Mg2+ (up to 3 mM) | Oryza sativa | |
| Mn2+ | the enzyme shows maximum activity at very low concentrations (less than 0.05 mM) of Mn2+ | Oryza sativa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Oryza sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.016 | - |
with sn-glycero-3-phosphoinositol as substrate, at pH 7.5 and 45°C | Oryza sativa |
| 0.108 | - |
with sn-glycero-3-phosphocholine as substrate, at pH 7.5 and 45°C | Oryza sativa |
| 0.126 | - |
with sn-glycero-3-phosphoethanolamine as substrate, at pH 7.5 and 45°C | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sn-glycero-3-phosphocholine + H2O | - |
Oryza sativa | choline + sn-glycerol 3-phosphate | - |
? | |
| sn-glycero-3-phosphoethanolamine + H2O | - |
Oryza sativa | sn-glycerol-3-phosphate + ethanolamine | - |
? | |
| sn-glycero-3-phosphoinositol + H2O | - |
Oryza sativa | sn-glycerol-3-phosphate + inositol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 423500, recombinant His6-tagged enzyme, SDS-PAGE | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDPD2 | - |
Oryza sativa |
| glycerophosphodiester phosphodiesterase | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
any rise in pH above 7.5 results in a decrease of enzyme activity | Oryza sativa |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Oryza sativa | the enzyme is transcriptionally induced by phosphate deficiency | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme plays a role in low phosphate acclimation in rice | Oryza sativa |
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