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Literature summary for 3.1.4.2 extracted from

  • Schmidl, S.R.; Otto, A.; Lluch-Senar, M.; Pinol, J.; Busse, J.; Becher, D.; Stuelke, J.
    A trigger enzyme in Mycoplasma pneumoniae: impact of the glycerophosphodiesterase GlpQ on virulence and gene expression (2011), PLoS Pathog., 7, e1002263.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.2

Cloned(Commentary)

Cloned (Comment) Organism
genes glpQ and mpn566, expression of N-terminally Strep-tagged enzymes Mycoplasma pneumoniae

Protein Variants

Protein Variants Comment Organism
additional information generationof GlpQ and of MPN566 knockout strains, phenotypes, overview. The MPN566-deficient strain does not show an altered phenotype Mycoplasma pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ no activity with Mycoplasma pneumoniae
Co2+ no activity with Mycoplasma pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates, required for activity Mycoplasma pneumoniae
Mn2+ activates, less activating than Mg2+ Mycoplasma pneumoniae
Zn2+ activates, less activating than Mg2+ Mycoplasma pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycero-3-phosphocholine + H2O Mycoplasma pneumoniae
-
 choline + sn-glycerol 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycoplasma pneumoniae
-
 gene glpQ
-

Purification (Commentary)

Purification (Comment) Organism
N-terminally Strep-tagged enzymes Mycoplasma pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycero-3-phosphocholine + H2O
-
 Mycoplasma pneumoniae choline + sn-glycerol 3-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
GlpQ
-
 Mycoplasma pneumoniae
glycerophosphodiesterase
-
 Mycoplasma pneumoniae
MPN420
-
 Mycoplasma pneumoniae

General Information

General Information Comment Organism
malfunction inactivation of the glpQ gene results in reduced growth in medium with glucose as the carbon source, in loss of hydrogen peroxide production when phosphatidylcholine is present, and in a complete loss of cytotoxicity towards HeLa cells, and the glpQ mutant strain exhibited a reduced gliding velocity, overview. The phenotype is reversible par complementation with the wild-type enzyme Mycoplasma pneumoniae
additional information a second glycerophosphodiesterase enzyme, MPN566, is catalytically inactive and its deletion does not cause an altered phenotype Mycoplasma pneumoniae
physiological function GlpQ is implicated in the control of gene expression, apparent regulation by GlpQ is exerted at the level of transcription. All genes subject to GlpQ-dependent control have a conserved potential cis-acting element upstream of the coding region. This element overlaps the promoter in the case of the genes that are repressed in a GlpQ-dependent manner and it is located upstream of the promoter for GlpQ-activated genes. GlpQ is central to the normal physiology and to pathogenicity of the minimal pathogen Mycoplasma pneumoniae