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Literature summary for 3.1.4.2 extracted from

  • Forsgren, A.; Riesbeck, K.; Janson, H.
    Protein D of Haemophilus influenzae: a protective nontypeable H. influenzae antigen and a carrier for pneumococcal conjugate vaccines (2008), Clin. Infect. Dis., 46, 726-731.
    View publication on PubMed

Application

Application Comment Organism
drug development possible vaccination, Protein D is immunogenic in human. Vaccine containing polysaccharides from 7 serotypes (each conjugated to CRM197, a nontoxic mutant of diphtheria toxin that is an immunogenically inactive carrier) shows high efficacy in young children. Experiments with rats: vaccination with Protein D induces high levels of serum IgG and IgA antibodies and significant bactericidal activity against homologous and heterologous strains (rat middle ear clearance model, rat pulmonary clearance model). Pronounced activity of Protein D antibodies is shown in the chinchilla acute otitis media model Haemophilus influenzae

Protein Variants

Protein Variants Comment Organism
additional information construction of isogenic Haemophilus influenzae mutant lacking the ability to express Protein D. effect on the middle ears of rats in an otitis model: Protein D is not essential for survival of Haemophilus influenzae in the middle ear of rats, Protein D enhances the severity of the disease, mutant effect on human nasopharyngeal tissue culture model: Protein D is involved in pathogenesis of upper respiratory tract infections due to NT Haemophilus influenzae, probably by enhancing functional and morphological damage to ciliated epithelial cells. NT Haemophilus influenzae probably avoids the immune system: Viable intracellular NT Haemophilus influenzae has been recovered from epithelial cells and macrophages from the adenoids of children undergoing adenoidectomy because of nasal obstruction, some strains can resist macrophage-mediated killing, Protein D expression promotes adherence and internalization of NT Haemophilus influenzae into human monocytic cells. Haemophilus influenzae

Localization

Localization Comment Organism GeneOntology No. Textmining
outer membrane outer membrane-anchored lipoprotein Haemophilus influenzae 19867
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41820
-
calculated from DNA sequence encoding Protein D (346 amino acids), SDS-PAGE, Western Blot Haemophilus influenzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycero-3-phosphocholine + H2O Haemophilus influenzae
-
sn-glycerol 3-phosphate + choline
-
?

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae
-
found in all 127 Haemophilus influenzae strains, including encapsulated serotypes a-f and NT Haemophilus influenzae strains, number of Protein D molecules is estimated to be 2800 molecules per bacterial cell
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information acylation of Protein D determines its hydrophobicity Haemophilus influenzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
enzymatic activity similar to that of GlpQ (glycerophosphodiester phosphodiesterase, EC 3.1.4.44) of Escherichia coli Haemophilus influenzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycero-3-phosphocholine + H2O
-
Haemophilus influenzae sn-glycerol 3-phosphate + choline
-
?

Synonyms

Synonyms Comment Organism
Protein D
-
Haemophilus influenzae