Application | Comment | Organism |
---|---|---|
biotechnology | efficient selection system for the identification of phosphotriesterase mutants with enhanced catalytic properties for selected organophosphate substrates | Klebsiella aerogenes |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
dimethyl phosphate | - |
Klebsiella aerogenes | |
0.4 | - |
4-nitrophenyl ethylphosphonate | - |
Klebsiella aerogenes | |
0.5 | - |
diethyl phosphate | - |
Klebsiella aerogenes | |
0.74 | - |
ethyl methylphosphonate | - |
Klebsiella aerogenes | |
0.9 | - |
bis(4-nitrophenyl) phosphate | - |
Klebsiella aerogenes | |
0.9 | - |
ethyl 4-nitrophenyl phosphate | - |
Klebsiella aerogenes | |
1.3 | - |
4-nitrophenyl methylphosphonate | - |
Klebsiella aerogenes | |
1.8 | - |
4-nitrophenyl phenyl phosphate | - |
Klebsiella aerogenes | |
2.6 | - |
methyl 4-nitrophenyl phosphate | - |
Klebsiella aerogenes | |
3.3 | - |
isobutyl methylphosphonate | - |
Klebsiella aerogenes | |
6.5 | - |
isopropyl 4-nitrophenyl phosphate | - |
Klebsiella aerogenes | |
13 | - |
4-nitrophenyl phenyl phosphonate | - |
Klebsiella aerogenes | |
19 | - |
isopropyl methylphosphonate | - |
Klebsiella aerogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Klebsiella aerogenes | |
Mn2+ | specific acitivity towards bis(4-nitrophenyl) phosphate is enhanced 4fold in presence of MnCl2 | Klebsiella aerogenes | |
Zn2+ | specific acitivity towards bis(4-nitrophenyl) phosphate is enhanced 2fold in presence of ZnCl2 | Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30840 | - |
calculated for one subunit | Klebsiella aerogenes |
31000 | - |
determined by SDS-PAGE | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
size exclusion column, anion-exchange column | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2,2-trimethylpropyl methylphosphonate + H2O | - |
Klebsiella aerogenes | 3,3-dimethylbutan-2-ol + methylphosphonate | - |
ir | |
4-nitrophenyl ethylphosphonate + H2O | - |
Klebsiella aerogenes | 4-nitrophenyl + ethylphosphonate | - |
ir | |
4-nitrophenyl methylphosphonate + H2O | - |
Klebsiella aerogenes | 4-nitrophenyl + methylphosphonate | - |
ir | |
4-nitrophenyl phenyl phosphate + H2O | - |
Klebsiella aerogenes | 4-nitrophenol + phenyl phosphate | - |
ir | |
4-nitrophenyl phenyl phosphonate + H2O | - |
Klebsiella aerogenes | 4-nitrophenol + phenyl phosphonate | - |
ir | |
4-nitrophenylphosphate + H2O | - |
Klebsiella aerogenes | 4-nitrophenol + phosphate | - |
ir | |
bis(4-nitrophenyl) phosphate + H2O | - |
Klebsiella aerogenes | 4-nitrophenol + phosphate | - |
ir | |
diethyl phosphate + H2O | - |
Klebsiella aerogenes | ethanol + ethyl phosphate | - |
ir | |
dimethyl 4-nitrophenyl phosphate + H2O | - |
Klebsiella aerogenes | ? | - |
ir | |
dimethyl phosphate + H2O | - |
Klebsiella aerogenes | methanol + methyl phosphate | - |
ir | |
ethyl 4-nitrophenyl phosphate + H2O | - |
Klebsiella aerogenes | 4-nitrophenol + ethyl phosphate | - |
ir | |
ethyl demeton S | - |
Klebsiella aerogenes | ? | - |
? | |
ethyl methyl 4-nitrophenyl phosphate + H2O | - |
Klebsiella aerogenes | ? | - |
ir | |
ethyl methylphosphonate + H2O | - |
Klebsiella aerogenes | ethanol + methylphosphonate | - |
ir | |
isobutyl methylphosphonate + H2O | - |
Klebsiella aerogenes | isobutanol + methylphosphonate | - |
ir | |
isopropyl 4-nitrophenyl phosphate + H2O | - |
Klebsiella aerogenes | 4-nitrophenol + isopropyl phosphate | - |
ir | |
isopropyl methylphosphonate + H2O | - |
Klebsiella aerogenes | isopropanol + methylphosphonate | - |
ir | |
methyl 4-nitrophenyl phosphate + H2O | - |
Klebsiella aerogenes | 4-nitrophenol + methyl phosphate | - |
ir | |
O-ethyl S-[2-(ethylsulfanyl)ethyl] hydrogen thiophosphate + H2O | - |
Klebsiella aerogenes | ? | - |
ir | |
paraoxon + H2O | - |
Klebsiella aerogenes | 4-nitrophenol + diethyl phosphate | - |
ir | |
phenyl methylphosphonate + H2O | - |
Klebsiella aerogenes | phenol + methylphosphonate | - |
ir | |
S-[2-(ethylsulfanyl)ethyl] O,O-dimethyl thiophosphate + H2O | - |
Klebsiella aerogenes | ? | - |
ir | |
S-[2-(ethylsulfanyl)ethyl] O-methyl hydrogen thiophosphate + H2O | - |
Klebsiella aerogenes | ? | - |
ir | |
S-[2-[bis(1-methylethyl)amino]ethyl] O-ethyl hydrogen thiophosphate + H2O | - |
Klebsiella aerogenes | ? | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
glycerophosphodiesterase | - |
Klebsiella aerogenes |
GpdQ | - |
Klebsiella aerogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
isopropyl methylphosphonate | - |
Klebsiella aerogenes | |
0.001 | - |
isobutyl methylphosphonate | - |
Klebsiella aerogenes | |
0.0012 | - |
ethyl methylphosphonate | - |
Klebsiella aerogenes | |
0.0012 | - |
O-ethyl S-[2-(ethylsulfanyl)ethyl] hydrogen thiophosphate | - |
Klebsiella aerogenes | |
0.0031 | - |
phenyl methylphosphonate | - |
Klebsiella aerogenes | |
0.005 | - |
dimethyl phosphate | - |
Klebsiella aerogenes | |
0.0074 | - |
diethyl phosphate | - |
Klebsiella aerogenes | |
0.5 | - |
4-nitrophenyl ethylphosphonate | - |
Klebsiella aerogenes | |
1 | - |
isopropyl 4-nitrophenyl phosphate | - |
Klebsiella aerogenes | |
1.1 | - |
S-[2-[bis(1-methylethyl)amino]ethyl] O-ethyl hydrogen thiophosphate | - |
Klebsiella aerogenes | |
1.6 | - |
4-nitrophenyl methylphosphonate | - |
Klebsiella aerogenes | |
2 | - |
ethyl 4-nitrophenyl phosphate | - |
Klebsiella aerogenes | |
2.8 | - |
4-nitrophenyl phenyl phosphonate | - |
Klebsiella aerogenes | |
4 | - |
4-nitrophenyl phenyl phosphate | - |
Klebsiella aerogenes | |
6 | - |
bis(4-nitrophenyl) phosphate | - |
Klebsiella aerogenes | |
7.3 | - |
methyl 4-nitrophenyl phosphate | - |
Klebsiella aerogenes |