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Literature summary for 3.1.4.17 extracted from
- Purification and characterization of cyclic GMP-stimulated cyclic nucleotide phosphodiesterase from calf liver. Effects of divalent cations on activity (1983), J. Biol. Chem., 258, 12526-12533.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | inhibits at concentrations higher than optimal | Bos taurus |  |
| Mg2+ | inhibits at concentrations higher than optimal | Bos taurus | |
| Mn2+ | inhibits at concentrations higher than optimal | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Bos taurus | |
| 0.015 | - |
3',5'-cGMP | - |
Bos taurus | |
| 0.033 | - |
3',5'-cAMP | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | stimulates | Bos taurus | |
| Mg2+ | stimulates | Bos taurus | |
| Mn2+ | stimulates | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 102000 | - |
SDS-PAGE | Bos taurus |
| 201000 | - |
sedimentation coefficient, Stokes radius | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 13900fold | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.33 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 15% glycerol, 5 mg/ml ovalbumin, stable for at least 4 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3',5'-cAMP + H2O | - |
Bos taurus | 5'-AMP | - |
? | |
| 3',5'-cGMP + H2O | - |
Bos taurus | 5'-GMP | - |
? | |
| nucleoside 3',5'-cyclic phosphate + H2O | - |
Bos taurus | nucleoside 5'-phosphate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Bos taurus | |
| 567 | - |
cAMP | stimulation with Mg2+ or Mn2+ | Bos taurus | |
| 667 | - |
cGMP | stimulation with Mg2+ or Mn2+ | Bos taurus | |
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