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Literature summary for 3.1.4.16 extracted from

  • Fernandez-Centeno, E.; Heredia, C.F.
    2',3'-Cyclic nucleotide 2'-phosphodiesterase from Fusarium culmorum (2000), Comp. Biochem. Physiol. B, 125, 161-167.
    View publication on PubMed
Search for more literature for 3.1.4.16

General Stability

General Stability Organism
activity is not affected by several cycles of freezing and thawing Fusarium culmorum

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 0.1 mM, 80% inhibition Fusarium culmorum
Cu2+ strong inhibition at micromolar concentrations, no effect on enzyme stability Fusarium culmorum
Mn2+ 0.5 mM, 50% inhibition Fusarium culmorum
additional information not inhibited by EDTA Fusarium culmorum
Zn2+ strong inhibition at micromolar concentrations, no effect on enzyme stability Fusarium culmorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 cyclic 2',3'-GMP pH 7.5, 30°C Fusarium culmorum
0.3
-
 cyclic 2',3'-AMP pH 7.5, 30°C Fusarium culmorum
0.6
-
 cyclic 2',3'-CMP pH 7.5, 30°C Fusarium culmorum
2
-
 cyclic 2',3'-UMP pH 7.5, 30°C Fusarium culmorum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for metal ions Fusarium culmorum

Organism

Organism UniProt Comment Textmining
Fusarium culmorum
-
 CECT 2148
-
Fusarium culmorum CECT 2148
-
 CECT 2148
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Fusarium culmorum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.025
-
 pH 7.5, 30°C, crude extract Fusarium culmorum

Storage Stability

Storage Stability Organism
-20°C, several weeks, stable Fusarium culmorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic 2',3'-AMP + H2O
-
 Fusarium culmorum 3'-AMP the only major reaction product ?
cyclic 2',3'-AMP + H2O
-
 Fusarium culmorum CECT 2148 3'-AMP the only major reaction product ?
cyclic 2',3'-CMP + H2O
-
 Fusarium culmorum 3'-CMP the only major reaction product ?
cyclic 2',3'-CMP + H2O
-
 Fusarium culmorum CECT 2148 3'-CMP the only major reaction product ?
cyclic 2',3'-GMP + H2O
-
 Fusarium culmorum 3'-GMP the only major reaction product ?
cyclic 2',3'-GMP + H2O
-
 Fusarium culmorum CECT 2148 3'-GMP the only major reaction product ?
cyclic 2',3'-UMP + H2O
-
 Fusarium culmorum 3'-UMP the only major reaction product ?
cyclic 2',3'-UMP + H2O
-
 Fusarium culmorum CECT 2148 3'-UMP the only major reaction product ?
additional information not: 3’,5’-nucleoside monophosphates, nucleoside 5’-, 3’- or 2’-monophosphates, tRNA, polyU Fusarium culmorum ?
-
 ?
additional information not: 3’,5’-nucleoside monophosphates, nucleoside 5’-, 3’- or 2’-monophosphates, tRNA, polyU Fusarium culmorum CECT 2148 ?
-
 ?
nucleoside 2',3'-cyclic phosphate + H2O
-
 Fusarium culmorum nucleoside 3'-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Fusarium culmorum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 80 very stable to heat, medium concentrate of enzyme, 5 min, stable Fusarium culmorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Fusarium culmorum

pH Range

pH Minimum pH Maximum Comment Organism
8
-
 at pH-values greater than 8 activity declines sharply Fusarium culmorum