Cloned (Comment) | Organism |
---|---|
expressed in Sf9 cells | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.2M lithium acetate and 20% (w/v) PEG 3350 | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
F388R | the mutant shows reduced activity with sphingomyelin and bis(4-nitrophenyl)phosphate as substrate as compared to the wild type enzyme | Mus musculus |
H280A | inactive with sphingomyelin and bis(4-nitrophenyl)phosphate as substrate | Mus musculus |
H317A | inactive with sphingomyelin as substrate, but active with bis(4-nitrophenyl)phosphate as substrate as compared to the wild type enzyme | Mus musculus |
L391R | the mutant shows about 20% activity with sphingomyelin and about 50% activity with bis(4-nitrophenyl)phosphate as substrate, as compared to the wild type enzyme | Mus musculus |
P321E | the mutant shows reduced activity with sphingomyelin and almost no activity with bis(4-nitrophenyl)phosphate as substrate, compared to the wild type enzyme | Mus musculus |
V128E | the mutant shows about 20% activity with sphingomyelin and about 70% activity with bis(4-nitrophenyl)phosphate as substrate as compared to the wild type enzyme | Mus musculus |
V143R | the mutant shows about 5% activity with sphingomyelin and about 65% activity with bis(4-nitrophenyl)phosphate as substrate as compared to the wild type enzyme | Mus musculus |
W283N | the mutant shows reduced activity with sphingomyelin and bis(4-nitrophenyl)phosphate as substrate as compared to the wild type enzyme | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-aminodecylidene bis-phosphonic acid | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Mus musculus | 5764 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains two zinc ions | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingomyelin + H2O | Mus musculus | - |
ceramide + phosphocholine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q04519 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the protein has five sites of N-glycosylation | Mus musculus |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Superdex 200 gel filtration | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bis(4-nitrophenyl)phosphate + H2O | - |
Mus musculus | ? | - |
? | |
sphingomyelin + H2O | - |
Mus musculus | ceramide + phosphocholine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
acid sphingomyelinase | - |
Mus musculus |
ASM | - |
Mus musculus |
aSMase | - |
Mus musculus |
SMPD1 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | inactivating enzyme mutations cause Niemann-Pick disease | Mus musculus |