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Literature summary for 3.1.4.12 extracted from

  • Gorelik, A.; Heinz, L.X.; Illes, K.; Superti-Furga, G.; Nagar, B.
    Crystal structure of the acid sphingomyelinase-like phosphodiesterase SMPDL3B provides insights into determinants of substrate specificity (2016), J. Biol. Chem., 291, 24054-24064 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 cells and RAW264.7 cells Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
apo-enzyme and in complex with phosphorylcholine, hanging drop vapor diffusion method, using 0.2 M NH4NO3 and 25% (w/v) PEG 3350 Mus musculus

Protein Variants

Protein Variants Comment Organism
H135A almost inactive Mus musculus
K140M the mutant shows reduced activity compared to the wild type enzyme Mus musculus
K140M/N141A almost inactive Mus musculus
N141A the mutant shows reduced activity compared to the wild type enzyme Mus musculus
N200A the mutant shows increased activity compared to the wild type enzyme Mus musculus
Y198F the mutant shows reduced activity compared to the wild type enzyme Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Mus musculus 9986
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains two Zn2+ ions Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P58242
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
 Mus musculus AMP + phosphate
-
 ?
ATP + H2O
-
 Mus musculus ADP + phosphate
-
 ?
bis(4-nitrophenyl)phosphate + H2O
-
 Mus musculus ?
-
 ?
CDP-choline + H2O
-
 Mus musculus CMP + phosphorylcholine
-
 ?
additional information no activity with AMP and diadenosine tetraphosphate Mus musculus ?
-
 ?
additional information the soluble enzyme form that lacks the GPI moiety responsible for anchoring SMPDL3B to the cell surface displays no head group hydrolysis with the following substrates: sphingomyelin in Triton X-100 micelles, sphingomyelin in neutral liposomes (also comprising phosphatidylcholine and cholesterol), the soluble micelle-forming lipids sphingosylphosphorylcholine, and platelet-activating factor Mus musculus ?
-
 ?
sphingomyelin + H2O
-
 Mus musculus ceramide + phosphorylcholine
-
 ?

Synonyms

Synonyms Comment Organism
acid sphingomyelinase-like phosphodiesterase 3B
-
 Mus musculus
SMPDL3B
-
 Mus musculus

General Information

General Information Comment Organism
physiological function the enzyme acts as a negative regulator of innate immune signaling, affecting cellular lipid composition and membrane fluidity Mus musculus