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Literature summary for 3.1.4.12 extracted from

  • Young, S.A.; Smith, T.K.
    The essential neutral sphingomyelinase is involved in the trafficking of the variant surface glycoprotein in the bloodstream form of Trypanosoma brucei (2010), Mol. Microbiol., 76, 1461-1482.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.12

Application

Application Comment Organism
drug development TbnSMase is therefore genetically validated as a drug target against African trypanosomes, and suggests that interfering with the endocytic transport of variant surface glycoprotein is a highly desirable strategy for drug development against African trypanosomasis Trypanosoma brucei

Cloned(Commentary)

Cloned (Comment) Organism
nSMase overexpression as GST-tagged enzyme in Escherichia coli strain C43 (DE3) Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Trypanosoma brucei 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
 x * 81000, recombinant GST-tagged nSMase, SDS-PAGE Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a sphingomyelin + H2O Trypanosoma brucei
-
 a ceramide + choline phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a sphingomyelin + H2O
-
 Trypanosoma brucei a ceramide + choline phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 81000, recombinant GST-tagged nSMase, SDS-PAGE Trypanosoma brucei

Synonyms

Synonyms Comment Organism
neutral sphingomyelinase
-
 Trypanosoma brucei

General Information

General Information Comment Organism
malfunction loss of nSMase activity leads to a Big-Eye-like phenotype, and affect s protein trafficking, as well as VSG trafficking, overview Trypanosoma brucei
metabolism the essential neutral sphingomyelinase is involved in the trafficking of the variant surface glycoprotein in the bloodstream form of Trypanosoma brucei Trypanosoma brucei
physiological function the neutral sphingomyelinase is essential for growth and survival of the parasite. The formation of ceramide in the endoplasmic reticulum affects post-Golgi sorting and rate of deposition of newly synthesized GPI-anchored variant surface glycoprotein on the cell surface Trypanosoma brucei