Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cholesterol | sphingomyelinase is enhanced by cholesterol and by lipids with an intrinsic negative curvature, e.g. phosphatidylethanolamine | Clostridium perfringens | |
additional information | no effect on activity by monosialic ganglioside GM3 and phospholipids | Clostridium perfringens | |
phosphatidylethanolamine | sphingomyelinase is enhanced by cholesterol and by lipids with an intrinsic negative curvature, e.g. phosphatidylethanolamine | Clostridium perfringens |
Cloned (Comment) | Organism |
---|---|
expression of alpha-toxin in Escherichia coli | Clostridium perfringens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ganglioside GT1b | - |
Clostridium perfringens | |
Lysophospholipids | - |
Clostridium perfringens | |
additional information | no effect on activity by monosialic ganglioside GM3 and phospholipids | Clostridium perfringens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a sphingomyelin + H2O | Clostridium perfringens | - |
a ceramide + choline phosphate | - |
? | |
a sphingomyelin + H2O | Clostridium perfringens 8-6 | - |
a ceramide + choline phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | - |
- |
- |
Clostridium perfringens 8-6 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a sphingomyelin + H2O | - |
Clostridium perfringens | a ceramide + choline phosphate | - |
? | |
a sphingomyelin + H2O | - |
Clostridium perfringens 8-6 | a ceramide + choline phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-toxin | - |
Clostridium perfringens |
SMase | - |
Clostridium perfringens |
sphingomyelinase | - |
Clostridium perfringens |
General Information | Comment | Organism |
---|---|---|
additional information | establishing enzyme activity in lipid vesicles, method development, overview. Both lipase activities are sensitive to vesicle size, but in opposite ways: while phospholipase C is higher with larger vesicles, sphingomyelinase activity is lower | Clostridium perfringens |
physiological function | alpha-toxin, a major determinant of Clostridium perfringens toxicity, exhibits both phospholipase C and sphingomyelinase activities with distinct, but partially overlapping and interacting active sites | Clostridium perfringens |