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Literature summary for 3.1.4.12 extracted from
- Purification and characterization of a membrane bound neutral pH optimum magnesium-dependent and phosphatidylserine-stimulated sphingomyelinase from rat brain (1998), J. Biol. Chem., 273, 34472-34479.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| arachidonic acid | - |
Rattus norvegicus |  |
| dithiothreitol | 2fold enhanced activity at 3 mM | Rattus norvegicus | |
| lyso-phosphatidylcholine | - |
Rattus norvegicus | |
| phosphatidic acid | - |
Rattus norvegicus | |
| phosphatidylethanolamine | - |
Rattus norvegicus | |
| phosphatidylinositol | - |
Rattus norvegicus | |
| phosphatidylserine | - |
Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Rattus norvegicus | |
| deoxycholate | - |
Rattus norvegicus | |
| glutathione | - |
Rattus norvegicus | |
| Mg2+ | slight inhibitory at higher concentrations | Rattus norvegicus | |
| Ni2+ | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
sphingomyelin | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent | Rattus norvegicus | |
| Mn2+ | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, 3030fold | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 14 days, Triton X-100 | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphingomyelin + H2O | - |
Rattus norvegicus | N-acylsphingosine + choline phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000-90000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
neutral sphingomyelinase | Rattus norvegicus |
| 7.5 | - |
- |
Rattus norvegicus |
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