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Literature summary for 3.1.4.12 extracted from
- Purification and characterization of sphingomyelinase from human brain (1977), J. Biol. Chem., 252, 3805-3813.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| albumin | 20% activation of enzyme A and B | Homo sapiens | |
| Triton X-100 | activation of enzyme A and B | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.095 | - |
sphingomyelin | enzyme B | Homo sapiens | |
| 0.3 | - |
sphingomyelin | enzyme A | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Homo sapiens | 5764 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5fold activation of enzyme A and B at pH 7.5 | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration, enzyme B | Homo sapiens |
| 150000 | - |
enzyme A, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphingomyelin + H2O | Homo sapiens | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme A 800fold pure, enzyme B 2500fold pure | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 10 mM Tris-HCl, pH 7.2, 0. 1% Triton X-100, 10 mM 2-mercaptoethanol, protein 0.1 mg/ml, more than 2 months | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphingomyelin + H2O | - |
Homo sapiens | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
up to | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
enzyme A and B | Homo sapiens |
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