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Literature summary for 3.1.4.11 extracted from

  • Khalil, H.B.; Wang, Z.; Wright, J.A.; Ralevski, A.; Donayo, A.O.; Gulick, P.J.
    Heterotrimeric Galpha subunit from wheat (Triticum aestivum), GA3, interacts with the calcium-binding protein, Clo3, and the phosphoinositide-specific phospholipase C, PI-PLC1 (2011), Plant Mol. Biol., 77, 145-158.
    View publication on PubMed
Search for more literature for 3.1.4.11

Activating Compound

Activating Compound Comment Organism Structure
activated Galpha proteins
-
 Triticum aestivum

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged PI-PLC1 in Escherichia coli strain BL21. Pi-Plc1 and Pi-Plc2, expression of full-length enzymes in Nicotiana benthamiana via Agrobacterium tunefaciens strain AGL1 transfection method, co-expression with GA3 and Clo3 Triticum aestivum

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane inner leaflet Triticum aestivum 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O Triticum aestivum
-
 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
 genes Pi-Plc1 and Pi-Plc2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged PI-PLC1 from Escherichia coli strain BL21 by nickel affinity chromatography Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Triticum aestivum 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?

Synonyms

Synonyms Comment Organism
phosphoinositide-specific phospholipase C
-
 Triticum aestivum
PI-PLC1
-
 Triticum aestivum

General Information

General Information Comment Organism
additional information protein-protein interactions between the wheat heterotrimeric Ga subunit, GA3, the calcium-binding protein, Clo3 and the phosphoinositide-specific phospholipase C, PI-PLC1, in vivo and in vitro, analysis, overview Triticum aestivum