Literature summary for 3.1.4.11 extracted from

Vossen, J.H.; Abd-El-Haliem, A.; Fradin, E.F.; van den Berg, G.C.; Ekengren, S.K.; Meijer, H.J.; Seifi, A.; Bai, Y.; ten Have, A.; Munnik, T.; Thomma, B.P.; Joosten, M.H.
Identification of tomato phosphatidylinositol-specific phospholipase-C (PI-PLC) family members and the role of PLC4 and PLC6 in HR and disease resistance (2010), Plant J., 62, 224-239.

Cloned(Commentary)

Cloned (Comment)	Organism
full-length PLC6 cDNA amplified, in-frame cloning of PLC6 into the pGEX-KG plasmid resulting in a GST-PLC6 fusion. For the GST-PLC4, PLC4 amplified from a plasmid containing full-length PLC4, cloned into pGEMT and then isolated by digestion with XbaI and XhoI. Both expressed in Escherichia coli strain BL21. PLC4 expression construct ligated into the XhoI and EcoRI restriction sites downstream of the 35S promoter of a pMOG800-based binary vector Nicotiana benthamiana	Solanum lycopersicum

Cloned (Comment)

Organism

full-length PLC6 cDNA amplified, in-frame cloning of PLC6 into the pGEX-KG plasmid resulting in a GST-PLC6 fusion. For the GST-PLC4, PLC4 amplified from a plasmid containing full-length PLC4, cloned into pGEMT and then isolated by digestion with XbaI and XhoI. Both expressed in Escherichia coli strain BL21. PLC4 expression construct ligated into the XhoI and EcoRI restriction sites downstream of the 35S promoter of a pMOG800-based binary vector Nicotiana benthamiana

Solanum lycopersicum

Organism

Organism	UniProt	Comment	Textmining
Solanum lycopersicum	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
seedling	-	Solanum lycopersicum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphatidylinositol 4,5-bisphosphate + H2O	PLC4 and PLC6 encode catalytically active enzymes that convert phosphatidylinositol into diacylglycerol	Solanum lycopersicum	diacylglycerol + 1D-myo-inositol 1,4,5-triphosphate	-	?

Synonyms

Synonyms	Comment	Organism
phosphatidylinositol-specific phospholipase-C	-	Solanum lycopersicum
PI-PLC	-	Solanum lycopersicum
PLC1	-	Solanum lycopersicum
PLC2	-	Solanum lycopersicum
PLC3	-	Solanum lycopersicum
PLC4	-	Solanum lycopersicum
PLC5	-	Solanum lycopersicum
PLC6	-	Solanum lycopersicum

Expression

Organism	Comment	Expression
Solanum lycopersicum	PLC5 transcripts show the lowest abundance in each organ. Expression of PLC5 is repressed in mock-treated plants. In incompatible interaction, increase is transient for PLC1 and PLC4, as their expression levels decrease again at day 10 to reach the same levels as in the mock-treated plants	down
Solanum lycopersicum	in mock-treated plants, PLC2, PLC3, PLC4 and PLC6 expression levels are relatively stable. In incompatible interaction, the expression levels of PLC3 and PLC6 are not significantly affected	additional information
Solanum lycopersicum	PLC3 is the most abundantly expressed PLC gene. Expression of PLC1 is induced in mock-treated plants. In incompatible interaction, levels of PLC1, PLC2, PLC4 and PLC5 transcripts significantly increase	up

General Information

General Information	Comment	Organism
malfunction	silencing of PLC4 impairs the race-specific effector Avr4 from the pathogenic fungus Cladosporium fulvum/Cladosporium fulvum-resistant Cf-4 tomato-induced hypersensitive response and results in increased colonisation of Cladosporium fulvum-resistant Cf-4 plants by Cladosporium fulvum expressing Avr4. Silencing of PLC6 does not affect hypersensitive response, whereas it causes increased colonisation of Cf-4 plants by the fungus	Solanum lycopersicum
physiological function	PLC isoforms are required for the hypersensitive response and disease resistance. PLC4 is specifically required for Cladosporium fulvum-resistant Cf-4 tomato function, while PLC6 may be a more general component of resistance protein signalling. PLC4 and PLC6 are involved in Cf-4-mediated resistance to Cladosporium fulvum. PLC6, but not PLC4, is also required for resistance to Verticillium dahliae, mediated by the transmembrane Ve1 resistance protein, and to Pseudomonas syringae, mediated by the intracellular Pto/Prf resistance protein couple of Pseudomonas syringae. Expression of the gene in Nicotiana benthamiana enhances the Avr4/Cf-4-induced hypersensitive response	Solanum lycopersicum