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Literature summary for 3.1.4.11 extracted from

  • Park, S.K.; Lee, J.R.; Lee, S.S.; Son, H.J.; Yoo, J.Y.; Moon, J.C.; Kwon, H.Y.; Lim, C.O.; Bahk, J.D.; Cho, M.J.; Lee, S.Y.
    Partial purification and properties of a phosphatidylinositol 4,5-bisphosphate hydrolyzing phospholipase C from the soluble fraction of soybean sprouts (2002), Mol. Cells, 13, 377-384.
    View publication on PubMed
Search for more literature for 3.1.4.11

Activating Compound

Activating Compound Comment Organism Structure
sodium deoxycholate activates from 0.05% to 0.08%, but severely inhibits activity above 0.08%, no activity at 0.3% Glycine max

Inhibitors

Inhibitors Comment Organism Structure
sodium deoxycholate activates from 0.05% to 0.08%, but severely inhibits activity above 0.08%, no activity at 0.3% Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Glycine max 5829
-
soluble
-
 Glycine max
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates, maximal activity at 0.01 mM Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 x * 64000, SDS-PAGE Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, 56.5fold Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
sprout
-
 Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.6
-
 pH 7, 37°C, hydrolysis of phosphatidylinositol 4,5-bisphosphate Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
-
 Glycine max 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
-
 ?
additional information not: phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine Glycine max ?
-
 ?
phosphatidylinositol + H2O
-
 Glycine max myo-inositol 1-phosphate + diacylglycerol
-
 ?
phosphatidylinositol 4-phosphate + H2O
-
 Glycine max 1D-myo-inositol 4,5-bisphosphate + diacylglycerol
-
 ?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
1-phosphatidyl-D-myo-inositol-4,5-bisphosphate inositoltrisphosphohydrolase
-
 Glycine max
monophosphatidylinositol phosphodiesterase
-
 Glycine max
phosphatidylinositol phospholipase C
-
 Glycine max
PI-PLC
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 maximal activity in the pH-range 6.5-7.5 with a peak at pH 7 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5 maximal activity in the pH-range 6.5-7.5 with a peak at pH 7 Glycine max