Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidic acid | stimulating | Apium graveolens Dulce Group |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | addition of EDTA completely inhibits the activity | Apium graveolens Dulce Group | |
additional information | dialysis of the Apium graveolens var. dulce extract against water completely inhibits the activity | Apium graveolens Dulce Group | |
phosphatidylcholine | slightly inhibited, 30% at concentrations equimolar with phosphatidylinositol | Apium graveolens Dulce Group |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Apium graveolens Dulce Group | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enzyme requires a bivalent cation, Ca2+ being the most effective activator | Apium graveolens Dulce Group | |
Mg2+ | calcium, manganese, magnesium and zinc ions, in decreasing order of effectiveness, all partly restore the activity of the dialysed enzyme at a concentration of 2 mM. In each case 4 mM cation is less effective than 2 mM | Apium graveolens Dulce Group | |
Mn2+ | calcium, manganese, magnesium and zinc ions, in decreasing order of effectiveness, all partly restore the activity of the dialysed enzyme at a concentration of 2 mM. In each case 4 mM cation is less effective than 2 mM | Apium graveolens Dulce Group | |
Zn2+ | calcium, manganese, magnesium and zinc ions, in decreasing order of effectiveness, all partly restore the activity of the dialysed enzyme at a concentration of 2 mM. In each case 4 mM cation is less effective than 2 mM | Apium graveolens Dulce Group |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Allium cepa | - |
- |
- |
Apium graveolens Dulce Group | - |
celery | - |
Brassica oleracea var. botrytis | - |
cauliflower | - |
Narcissus pseudonarcissus | - |
daffodil | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | tips of the flowers | Brassica oleracea var. botrytis | - |
leaf | outer leaves | Allium cepa | - |
stem | the elongating stem | Narcissus pseudonarcissus | - |
stem | the lower regions of the stem | Apium graveolens Dulce Group | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
at a substrate concentration of 50 microM the rate of hydrolysis is 0.3 nmol/h per mg of tissue (wet weight) at pH 6.6 | Apium graveolens Dulce Group |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no detectable activity against phosphatidylcholine or phosphatidylethanolamine | Apium graveolens Dulce Group | ? | - |
? | |
phosphatidylinositol + H2O | - |
Apium graveolens Dulce Group | diacylglycerol + 1D-myo-inositol 1-phosphate | the lipoidal product of hydrolysis is diacylglycerol, the water-soluble products are 1D-myo-inositol 1,2-cyclic phosphate and 1D-myo-inositol 1-phosphate in the approximate proportions of 60% and 40%, respectively | ? |
Synonyms | Comment | Organism |
---|---|---|
phosphatidylinositol phosphodiesterase | - |
Narcissus pseudonarcissus |
phosphatidylinositol phosphodiesterase | - |
Allium cepa |
phosphatidylinositol phosphodiesterase | - |
Brassica oleracea var. botrytis |
phosphatidylinositol phosphodiesterase | - |
Apium graveolens Dulce Group |
phospholipase C-like phosphodiesterase | - |
Apium graveolens Dulce Group |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | 6.6 | - |
Apium graveolens Dulce Group |