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Literature summary for 3.1.4.11 extracted from
- Expression, purification and characterisation of a functional phosphatidylinositol-specific phospholipase C-delta1 protein in Escherichia coli (1992), Eur. J. Biochem., 210, 155-160.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylethanolamine | stimulation in presence of 1 mM Ca2+ | Homo sapiens |  |
| phosphatidylserine | stimulation in presence of 1 mM Ca2+ | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidylethanolamine | 25% inhibition, in presence of 0.001 mM Ca2+ | Homo sapiens | |
| phosphatidylserine | 50% inhibition, in presence of 0.001 mM Ca2+ | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
phosphatidylinositol 4,5-bisphosphate | - |
Homo sapiens | |
| 0.031 | - |
phosphatidylinositol 4-phosphate | - |
Homo sapiens | |
| 0.143 | - |
phosphatidylinositol | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | significant hydrolysis of phosphatidylinositol only above 0.1 mM | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 200 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylinositol + H2O | 7% of the activity with phosphatidylinositol 4,5-diphosphate | Homo sapiens | ? | - |
? | |
| phosphatidylinositol 4,5-bisphosphate + H2O | - |
Homo sapiens | D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
| phosphatidylinositol 4-phosphate | 20% of the activity with phosphatidylinositol 4,5-diphosphate | Homo sapiens | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | pH 7.0: about 30% of maximal activity, pH 7.5: about 75% of maximal activity, hydrolysis of phosphatidylinositol 4,5-diphosphate | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
phosphatidylserine | - |
Homo sapiens | |
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