Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 0.5 mM, 70% residual activity | Rattus norvegicus | |
cAMP | 3 mM, 86% residual activity | Rattus norvegicus | |
EDTA | complete inhibition, addition of EDTA plus Ca2+ or Mn2+ results in partial inhibition | Rattus norvegicus | |
additional information | not inhibitory: ouabain, N-ethylmaleimide, levamisole, sodium azide | Rattus norvegicus | |
phenylalanine | 5 mM, 87% residual activity | Rattus norvegicus | |
suramin | 0.25 mM, 87% residual activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.281 | - |
p-nitrophenyl 5'-thymidine monophosphate | pH 7.4, 37°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | or Mg2+, basal level required, no significant increase in activity by addition of 0.1 mM Ca2+ or Mg2+ | Rattus norvegicus | |
Mg2+ | or Ca2+, basal level required, no significant increase in activity by addition of 0.1 mM Ca2+ or Mg2+ | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
salivary gland | primary cell culture of submandibular salivary gland, co-localization of enzyme with an ecto-ATP diphosphohydrolase and an ecto-5'-nucleotidase | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl 5'-thymidine monophosphate + H2O | - |
Rattus norvegicus | p-nitrophenol + 5'-thymidine monophosphate | - |
? |