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Literature summary for 3.1.4.1 extracted from

  • Chen, S.; Yakunin, A.F.; Kuznetsova, E.; Busso, D.; Pufan, R.; Proudfoot, M.; Kim, R.; Kim, S.H.
    Structural and Functional Characterization of a Novel Phosphodiesterase from Methanococcus jannaschii (2004), J. Biol. Chem., 279, 31854-31862.
    View publication on PubMed
Search for more literature for 3.1.4.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Methanocaldococcus jannaschii

General Stability

General Stability Organism
stable Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
PO43- competitive Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 p-nitrophenyl 5'-thymidine monophosphate +/- 0.003, with 1 mM Ni2+ Methanocaldococcus jannaschii
0.15
-
 bis-p-nitrophenyl phosphate +/- 0.01, with 1 mM Ni2+ Methanocaldococcus jannaschii
0.66
-
 bis-p-nitrophenyl phosphate +/- 0.09, with 20 mM PO43- and 1 mM Ni2+ Methanocaldococcus jannaschii
1.01
-
 bis-p-nitrophenyl phosphate +/- 0.13, with 50 mM PO43- and 1 mM Ni2+ Methanocaldococcus jannaschii
1.53
-
 p-Nitrophenylphosphorylcholine +/- 0.1, with 20 mM Mn2+ Methanocaldococcus jannaschii
3.33
-
 p-Nitrophenylphosphorylcholine +/- 0.5, with 1 mM Ni2+ Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates, KD: 1.3 +/- 0.1 uM Methanocaldococcus jannaschii
Ni2+ activates, KD: 0.28 +/- 0.09 mM Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58346
-
-

Storage Stability

Storage Stability Organism
4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bis-p-nitrophenyl phosphate + H2O
-
 Methanocaldococcus jannaschii p-nitrophenol + p-nitrophenyl phosphate
-
 ?
p-nitrophenyl 5'-thymidine monophosphate + H2O
-
 Methanocaldococcus jannaschii 5'-TMP + p-nitrophenol
-
 ?
p-nitrophenylphosphorylcholine + H2O
-
 Methanocaldococcus jannaschii p-nitrophenol + phosphorylcholine
-
 ?

Subunits

Subunits Comment Organism
monomer crystallization Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
phosphodiesterase
-
 Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.26
-
 p-nitrophenyl 5'-thymidine monophosphate +/- 0.006, with 1mM Ni2+ Methanocaldococcus jannaschii
8.6
-
 p-Nitrophenylphosphorylcholine +/- 0.5, with 1 mM Ni2+ Methanocaldococcus jannaschii
52.9
-
 p-Nitrophenylphosphorylcholine +/- 1.0, with 20 mM Mn2+ Methanocaldococcus jannaschii
105.7
-
 bis-p-nitrophenyl phosphate +/- 2.8, with 1 mM Ni2+ Methanocaldococcus jannaschii
125.9
-
 bis-p-nitrophenyl phosphate +/- 8.1, with 20 mM PO43- and 1 mM Ni2+ Methanocaldococcus jannaschii
130.2
-
 bis-p-nitrophenyl phosphate +/- 8.6, with 50 mM PO43- and 1 mM Ni2+ Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4 9.8
-
 Methanocaldococcus jannaschii