Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.1 extracted from

  • Kurosawa, S.i.; Hayashi, M.; Ishizawa, H.
    Purification and properties of alkaline phosphodiesterase from the fruit body of Flammulina velutipes (1984), Agric. Biol. Chem., 48, 299-305.
No PubMed abstract available
Search for more literature for 3.1.4.1

General Stability

General Stability Organism
Zn2+ stabilizes, enzyme is very unstable in the absence of Zn2+ Flammulina velutipes

Inhibitors

Inhibitors Comment Organism Structure
EDTA 91% inhibition; inactivation reversed by Zn2+ Flammulina velutipes
o-phenanthroline 99% inhibition Flammulina velutipes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 4-nitrophenyl deoxythymidine 5'-phosphat
-
 Flammulina velutipes
0.25
-
 bis(p-nitrophenyl) phosphate
-
 Flammulina velutipes

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ low concentration, 0.05 mM, 23% activation Flammulina velutipes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 gel filtration Flammulina velutipes

Organism

Organism UniProt Comment Textmining
Flammulina velutipes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Flammulina velutipes

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
 Flammulina velutipes
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.65
-
-
 Flammulina velutipes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl deoxythymidine 5'-phosphate + H2O
-
 Flammulina velutipes 4-nitrophenol + deoxythymidine 5'-phosphate
-
 ?
5'-p-nitrophenyl deoxythymidine 5'-phosphate + H2O
-
 Flammulina velutipes 5'-dTMP + p-nitrophenol
-
 ?
adenylyl-3',5'-uridine + H2O
-
 Flammulina velutipes 5'-UMP + adenosine
-
 ?
ADP-ribose + H2O
-
 Flammulina velutipes 5'-AMP + ribosyl monophosphate
-
 ?
ApA + H2O
-
 Flammulina velutipes 5'-AMP + adenosine
-
 ?
bis(p-nitrophenyl) phosphate + H2O
-
 Flammulina velutipes ?
-
 ?
cyclic 3',5'-mononucleotides + H2O cyclic AMP: not a substrate Flammulina velutipes mononucleoside 5'-phosphate
-
 ?
dinucleotides + H2O
-
 Flammulina velutipes ?
-
 ?
additional information no activity with cyclic nucleotides such as 2',3'-cyclic AMP, 3',5'-cyclic AMP, or 3',5'-cyclic GMP Flammulina velutipes ?
-
 ?
NAD+ + H2O
-
 Flammulina velutipes 5'-AMP + NMN
-
 ?
oligonucleotides + H2O
-
 Flammulina velutipes oligonucleotides(n-1) + 5'-mononucleotides
-
 ?
UDP-glucose + H2O
-
 Flammulina velutipes glucose-1-phosphate + 5'-UMP
-
 ?

Synonyms

Synonyms Comment Organism
alkaline phosphodiesterase
-
 Flammulina velutipes
PDase
-
 Flammulina velutipes
phosphoric diester hydrolase
-
 Flammulina velutipes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 below, stable for 30 min. below 40øC Flammulina velutipes
50
-
 15 min at 50°C: activity completely lost Flammulina velutipes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Flammulina velutipes

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 6.5 stable between Flammulina velutipes