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Literature summary for 3.1.4.1 extracted from
- Purification and characterization of a novel bifunctional enzyme, cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase, from Aspergillus niger (1994), Biosci. Biotechnol. Biochem., 58, 250-255.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | FS-44: 5'-PDase activity of bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger |  |
| Cu2+ | FS-44: 5'-PDase activity of bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | |
| diisopropyl fluorophosphate | bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | |
| Fe2+ | bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | |
| Fe3+ | bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase, 50% inhibition | Aspergillus niger | |
| Hg2+ | FS-44: 5'-PDase activity of bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | |
| Mn2+ | FS-44: 5'-PDase activity of bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | |
| phenylmethylsulfonyl fluoride | FS-44: 5'-PDase activity of bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | |
| Zn2+ | FS-44: 5'-PDase activity of bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.96 | - |
5'-p-nitrophenyladenosine 5'-phosphate | FS-44: 5'-PDase activity of bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase, gel filtration and SDS-PAGE | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
FS-44, 5'-PDase hyperproductive mutant: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase, containing N-linked sugar chains, containing a beta-aspartyl-glycosylamine bond between innermost N-acetylglucosamine and asparagine residue | Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3230 | - |
FS-44: 5'-PDase activity of bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-p-nitrophenyladenosine 5'-phosphate + H2O | FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | 5'-AMP + p-nitrophenol | - |
? | |
| 5'-p-nitrophenylnucleoside 5'-monophosphate + H2O | - |
Aspergillus niger | nucleoside 5'-monophosphate + p-nitrophenol | - |
? | |
| adenylyl-2',5'-adenosine + H2O | FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | ? | - |
? | |
| adenylyl-3',5'-adenosine + H2O | FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | adenosine + 5'-AMP | - |
? | |
| adenylyl-3',5'-cytidine + H2O | FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | adenosine 3'-phosphate + cytidine | - |
? | |
| adenylyl-3',5'-uridine + H2O | FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | ? | - |
? | |
| cytidylyl-3',5'-adenosine + H2O | FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | ? | - |
? | |
| dinucleoside monophosphates + H2O | - |
Aspergillus niger | mononucleoside 5'-phosphate | - |
? | |
| dinucleotides + H2O | 3'-hydroxyl-terminated 3'-5'and 2'-5' linkage without specificity toward bases or sugars | Aspergillus niger | ? | - |
? | |
| guanylyl-3',5'-adenosine + H2O | FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | ? | - |
? | |
| oligonucleotides + H2O | - |
Aspergillus niger | oligonucleotides(n-1) + 5'-mononucleotides | - |
? | |
| uridylyl-3',5'-adenosine + H2O | FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 62000, FS-44 : bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5'-PDase | - |
Aspergillus niger |
| cRNPMase-5'-PDase | FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger |
| cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase I | FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
FS-44, 5'-PDase activity of bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase, stable up to | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
FS-44, 5'-PDase activity of bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase | Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 6 | FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase, stable between | Aspergillus niger |
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