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Literature summary for 3.1.4.1 extracted from
- Characterization of over-expressed alkaline phosphodiesterase I in tumor-derived fibroblasts from patients with neurofibromatosis (1993), Cell Biochem. Funct., 11, 271-277.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| nicotinamide | patients with neurofibromatosis, induction of alkaline phosphodiesterase I | Homo sapiens |  |
General Stability
| General Stability | Organism |
|---|---|
| enzyme extracted with Triton-X-100 is unstable | Homo sapiens |
| glycerol, 15%, stabilizes | Homo sapiens |
| Zn2+ stabilizes | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Homo sapiens | |
| EDTA | - |
Homo sapiens | |
| GTP | competitive | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight activation | Homo sapiens | |
| Mg2+ | slight activation | Homo sapiens | |
| Zn2+ | required for activity | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 125000 | - |
x * 125000, SDS-PAGE | Homo sapiens |
| 430000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
patients with neurofibromatosis: over-expression of alkaline phosphodiesterase I | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, patients with neurofibromatosis | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fibroblast | tumor-derived and normal fibroblasts, patients with neurofibromatosis | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.1 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | enzyme hydolyzes single-stranded DNA more preferentially than double-stranded DNA, the enzyme also hydrolyzes nicked superhelical covalently closed circular phiX174RFI DNA to yield first open circular DNA and then linear DNA | Homo sapiens | DNA(n-1) + 2'-deoxynucleoside 5'-phosphate | - |
? | |
| GTP + H2O | - |
Homo sapiens | ? | - |
? | |
| p-nitrophenylthymidine 5'-phosphate + H2O | - |
Homo sapiens | 5'-TMP + p-nitrophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 125000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline phosphodiesterase | alkaline phosphodiesterase I | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme very heat labile without Zn2+ | Homo sapiens |
| 60 | - |
enzyme preincubated with Zn2+, 50% of activity remained at, in the absence of Zn2+, no activity remained at | Homo sapiens |
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