Activating Compound | Comment | Organism | Structure |
---|---|---|---|
nicotinamide | patients with neurofibromatosis, induction of alkaline phosphodiesterase I | Homo sapiens |
General Stability | Organism |
---|---|
enzyme extracted with Triton-X-100 is unstable | Homo sapiens |
glycerol, 15%, stabilizes | Homo sapiens |
Zn2+ stabilizes | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Homo sapiens | |
EDTA | - |
Homo sapiens | |
GTP | competitive | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic membrane | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activation | Homo sapiens | |
Mg2+ | slight activation | Homo sapiens | |
Zn2+ | required for activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
125000 | - |
x * 125000, SDS-PAGE | Homo sapiens |
430000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
patients with neurofibromatosis: over-expression of alkaline phosphodiesterase I | - |
Purification (Comment) | Organism |
---|---|
partial, patients with neurofibromatosis | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | tumor-derived and normal fibroblasts, patients with neurofibromatosis | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | enzyme hydolyzes single-stranded DNA more preferentially than double-stranded DNA, the enzyme also hydrolyzes nicked superhelical covalently closed circular phiX174RFI DNA to yield first open circular DNA and then linear DNA | Homo sapiens | DNA(n-1) + 2'-deoxynucleoside 5'-phosphate | - |
? | |
GTP + H2O | - |
Homo sapiens | ? | - |
? | |
p-nitrophenylthymidine 5'-phosphate + H2O | - |
Homo sapiens | 5'-TMP + p-nitrophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 125000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphodiesterase | alkaline phosphodiesterase I | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme very heat labile without Zn2+ | Homo sapiens |
60 | - |
enzyme preincubated with Zn2+, 50% of activity remained at, in the absence of Zn2+, no activity remained at | Homo sapiens |