Literature summary for 3.1.31.1 extracted from

Tremillon, N.; Issaly, N.; Mozo, J.; Duvignau, T.; Ginisty, H.; Devic, E.; Poquet, I.
Production and purification of staphylococcal nuclease in Lactococcus lactis using a new expression-secretion system and a pH-regulated mini-reactor (2010), Microb. Cell Fact., 9, 37.

Application

Application	Comment	Organism
molecular biology	purified enzyme can be used as an exogenous reagent to clear cellular extracts and improve protein purification	Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment)	Organism
the enzyme is cloned into an expression-secretion vector lacking its own signal peptide but being fused to a lactococcal sequence encoding a signal peptide. It is then fused to a lactococcal sequence encoding a signal peptide. Functional recombinant expression of the enzyme under the control of a lactococcal promoter, that is inducible by zinc starvation, in Lactococcus lactis subsp. cremoris model strain MG1363, the enzyme is secreted to the GM17v culture medium, expression method optimization, overview	Staphylococcus aureus

Cloned (Comment)

Organism

the enzyme is cloned into an expression-secretion vector lacking its own signal peptide but being fused to a lactococcal sequence encoding a signal peptide. It is then fused to a lactococcal sequence encoding a signal peptide. Functional recombinant expression of the enzyme under the control of a lactococcal promoter, that is inducible by zinc starvation, in Lactococcus lactis subsp. cremoris model strain MG1363, the enzyme is secreted to the GM17v culture medium, expression method optimization, overview

Staphylococcus aureus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme is secreted	Staphylococcus aureus	-	-

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Lactococcus lactis cell culture medium by cation exchange chromatography	Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2000	-	purified recombinant enzyme, pH and temperature not specified in the publication	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
Nuc	-	Staphylococcus aureus
SNase	-	Staphylococcus aureus
staphylococcal nuclease	-	Staphylococcus aureus
thermonuclease	-	Staphylococcus aureus