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Literature summary for 3.1.31.1 extracted from

  • Schlessman, J.L.; Garcia-Moreno, B.E.
    Molecular determinants of the pKa values of Asp and Glu residues in staphylococcal nuclease (2009), Proteins, 77, 570-588.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
a hyperstable, acid-resistant form of SNase known as delta+PHS is expressed in Escherichia coli BL21/DE3 cells Staphylococcus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of the hyperstable mutant enzyme delta+PHS are grown using hanging drop vapor-diffusion methods at 4°C from a solution containing 17% 2-methyl-2,4-pentanediol, 2 M CaCl2, 3 M thymine-3',5'-diphosphate, and 25 mM potassium phosphate buffer, pH 8.0 Staphylococcus sp.

Protein Variants

Protein Variants Comment Organism
G50F/V51N/P117G/H124L/S128A/DELTA44-49 hyperstable, acid-resistant mutant form of enzyme known as delta+PHS Staphylococcus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the carboxylic groups in the active site of SNase are known to bind Ca2+ Staphylococcus sp.

Organism

Organism UniProt Comment Textmining
Staphylococcus sp.
-
-
-

Synonyms

Synonyms Comment Organism
SNase
-
Staphylococcus sp.
staphylococcal nuclease
-
Staphylococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
2.5 8 the overall secondary and tertiary structure of SNase remains unchanged between pH 2.5 and 8.0 Staphylococcus sp.