Literature summary for 3.1.31.1 extracted from

Sunil, S.; Hossain, M.J.; Ramasamy, G.; Malhotra, P.
Transient silencing of Plasmodium falciparum Tudor Staphylococcal Nuclease suggests an essential role for the protein (2008), Biochem. Biophys. Res. Commun., 372, 373-378.

Application

Application	Comment	Organism
medicine	possible target for the anti-malarial therapy tested by RNAi	Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment)	Organism
possible target for the anti-malarial therapy tested by RNAi. Role of PfTSN at asexual blood stages investigated by treatment of synchronized parasites at late ring stage with siRNA (analysed by manual counting and by morphological examination 40 h after treatment, additionally analyzed by real time PCR and immunofluoresence), 50 microg/ml concentration of siRNA: over 50% reduction in parasitemia observed, 100 microg/ml concentration of PfTSN siRNA: 60-70% reduction in parasitemia. Effect of PfTSN siRNA over the course of erythrocytic cycle investigated by treatment of parasites with PfTSN siRNA at late ring stage and morphologically examined after the treatment: after 2-3 hours abnormalities (vacuolation, size of vacuols seems to increase over time), after 12 hours most parasites are dead	Plasmodium falciparum

Cloned (Comment)

Organism

possible target for the anti-malarial therapy tested by RNAi. Role of PfTSN at asexual blood stages investigated by treatment of synchronized parasites at late ring stage with siRNA (analysed by manual counting and by morphological examination 40 h after treatment, additionally analyzed by real time PCR and immunofluoresence), 50 microg/ml concentration of siRNA: over 50% reduction in parasitemia observed, 100 microg/ml concentration of PfTSN siRNA: 60-70% reduction in parasitemia. Effect of PfTSN siRNA over the course of erythrocytic cycle investigated by treatment of parasites with PfTSN siRNA at late ring stage and morphologically examined after the treatment: after 2-3 hours abnormalities (vacuolation, size of vacuols seems to increase over time), after 12 hours most parasites are dead

Plasmodium falciparum

Inhibitors

Inhibitors	Comment	Organism	Structure
2'-deoxythymidine 3',5'-diphosphate	at 100 microM concentration of pdTp, parasites show block in development, most of the parasites appear dead or shrunken within six hours after inhibitor treatment	Plasmodium falciparum

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	-	-	-

Synonyms

Synonyms	Comment	Organism
PfTSN	-	Plasmodium falciparum
tudor staphylococcal nuclease	-	Plasmodium falciparum

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Release 2023.1 (January 2023)