Application | Comment | Organism |
---|---|---|
drug development | attractive potential target for the development of anti-malarial drugs | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
expression of protein fragment results in the formation of insoluble inclusion bodies, successful expression as fusion protein with Maltose Binding Protein | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3',5'-deoxythymidine diphosphate | addition to the cell culture medium blocks further growth | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | during trophozoite and schizont stages parts of enzyme localized in the cytoplasm | Plasmodium falciparum | 5737 | - |
nucleus | mainly nuclear localization | Plasmodium falciparum | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
x * 95000, SDS-PAGE of the recombinant fusion protein with Maltose Binding Protein | Plasmodium falciparum |
110000 | - |
x * 110000, SDS-PAGE, native protein | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Plasmodium falciparum | essential enzyme in the life cycle of Plasmodium falciparum | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
strains 3D7 and W2mef, enzyme expressed in all three blood stages: ring, trophozoite and schizont stage | - |
Purification (Comment) | Organism |
---|---|
recombinant fusion protein with Maltose Binding Protein | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GFP-ssDNA + H2O | - |
Plasmodium falciparum | ? | partial degradation | ? | |
GFP-ssRNA + H2O | - |
Plasmodium falciparum | ? | complete degradation | ? | |
M13mp18 DNA + H2O | circular single stranded DNA | Plasmodium falciparum | ? | - |
? | |
additional information | essential enzyme in the life cycle of Plasmodium falciparum | Plasmodium falciparum | ? | - |
? | |
additional information | GFP-dsDNA and GFP-dsRNA are not used as substrates | Plasmodium falciparum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 110000, SDS-PAGE, native protein | Plasmodium falciparum |
? | x * 95000, SDS-PAGE of the recombinant fusion protein with Maltose Binding Protein | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
PfTSN | - |
Plasmodium falciparum |
Staphylococcus aureus nuclease homologue | - |
Plasmodium falciparum |
TSN | - |
Plasmodium falciparum |
tudor staphylococcal nuclease | - |
Plasmodium falciparum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.05 | - |
after 48 h | Plasmodium falciparum | 3',5'-deoxythymidine diphosphate |