Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | competitive | Staphylococcus aureus | |
Cu2+ | competitive | Staphylococcus aureus | |
Hg2+ | competitive | Staphylococcus aureus | |
Mononucleotides | with 5'-phosphate end group | Staphylococcus aureus | |
oligonucleotides | with 5'-phosphate end group | Staphylococcus aureus | |
Zn2+ | competitive | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Staphylococcus aureus | |
Cu2+ | minimal activation if Ca2+ is replaced by Cu2+ | Staphylococcus aureus | |
Fe2+ | minimal activation if Ca2+ is replaced by Fe2+ | Staphylococcus aureus | |
Sr2+ | DNase but no RNase activity if Ca2+ is replaced Sr2+ | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus Foggi Worthington | - |
- |
- |
Staphylococcus aureus V8 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products | mechanism | Staphylococcus aureus |
Renatured (Comment) | Organism |
---|---|
high concentration of urea and guanidinium chloride denaturation is completely reversible at pH 3.5-3.7, 55-65°C | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-O-acetylnitrophenyl-pdT + H2O | - |
Staphylococcus aureus | ? | - |
? | |
5'-chloromethyl-pdTp-nitrophenyl + H2O | - |
Staphylococcus aureus | ? | - |
? | |
5'-O-acetyl-dTp-nitrophenyl + H2O | - |
Staphylococcus aureus | ? | - |
? | |
5'-sulfate-dTp-nitrophenyl + H2O | - |
Staphylococcus aureus | ? | - |
? | |
DNA + H2O | in native DNA Xp-dTp and Xp-dAp bonds are preferentially attached in denaturated DNA random cleavage | Staphylococcus aureus | 3'-deoxymononucleotides + dinucleotides | - |
? | |
DNA + H2O | denaturated DNA is hydrolyzed more rapidly than native DNA | Staphylococcus aureus | 3'-deoxymononucleotides + dinucleotides | - |
? | |
DNA + H2O | in native DNA Xp-dTp and Xp-dAp bonds are preferentially attached in denaturated DNA random cleavage | Staphylococcus aureus V8 | 3'-deoxymononucleotides + dinucleotides | - |
? | |
DNA + H2O | denaturated DNA is hydrolyzed more rapidly than native DNA | Staphylococcus aureus V8 | 3'-deoxymononucleotides + dinucleotides | - |
? | |
DNA + H2O | in native DNA Xp-dTp and Xp-dAp bonds are preferentially attached in denaturated DNA random cleavage | Staphylococcus aureus Foggi Worthington | 3'-deoxymononucleotides + dinucleotides | - |
? | |
DNA + H2O | denaturated DNA is hydrolyzed more rapidly than native DNA | Staphylococcus aureus Foggi Worthington | 3'-deoxymononucleotides + dinucleotides | - |
? | |
dTp-nitrophenyl + H2O | - |
Staphylococcus aureus | ? | - |
? | |
methyl-pdTp-nitrophenyl + H2O | - |
Staphylococcus aureus | ? | - |
? | |
additional information | specificity | Staphylococcus aureus | ? | - |
? | |
additional information | specificity | Staphylococcus aureus V8 | ? | - |
? | |
additional information | specificity | Staphylococcus aureus Foggi Worthington | ? | - |
? | |
nitrophenyl-pdT + H2O | - |
Staphylococcus aureus | ? | - |
? | |
nitrophenyl-pdT + H2O | - |
Staphylococcus aureus V8 | ? | - |
? | |
nitrophenyl-pdT + H2O | - |
Staphylococcus aureus Foggi Worthington | ? | - |
? | |
nitrophenyl-pdTp + H2O | - |
Staphylococcus aureus | ? | - |
? | |
nitrophenyl-pdTpdTp-nitrophenyl + H2O | - |
Staphylococcus aureus | ? | - |
? | |
RNA + H2O | - |
Staphylococcus aureus | nucleoside 3'-phosphates + dinucleotides | - |
? | |
RNA + H2O | - |
Staphylococcus aureus V8 | nucleoside 3'-phosphates + dinucleotides | - |
? | |
RNA + H2O | - |
Staphylococcus aureus Foggi Worthington | nucleoside 3'-phosphates + dinucleotides | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH optimum depends on Ca2+ concentration | Staphylococcus aureus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | depends on Ca2+ concentration | Staphylococcus aureus |