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Literature summary for 3.1.30.2 extracted from

  • Laquel-Robert, P.; Sellem, C.H.; Sainsard-Chanet, A.; Castroviejo, M.
    Identification and biochemical analysis of a mitochondrial endonuclease of Podospora anserina related to curved-DNA binding proteins (2007), Biochim. Biophys. Acta, 1770, 527-542.
    View publication on PubMed
Search for more literature for 3.1.30.2

Activating Compound

Activating Compound Comment Organism Structure
additional information weak stimulation by addition of recombinant human PCNA Podospora anserina

Cloned(Commentary)

Cloned (Comment) Organism
His-tag fusion protein expressed in Escherichia coli Podospora anserina

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ weak inhibition at 10 mM, 50% inhibition at 50 mM Podospora anserina
EDTA inactive in the presence of EDTA Podospora anserina
KCl weak inhibition up to 200 mM Podospora anserina
NaCl 10fold inhibition at 25 mM Podospora anserina

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Podospora anserina 5739
-
soluble N-terminal His-tagged protein mainly found in the culture supernatant Podospora anserina
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum concentration 10 mM, inhibitory at higher concentrations Podospora anserina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 gel filtration, SDS-PAGE Podospora anserina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dsDNA + H2O Podospora anserina
-
 ?
-
 ?
flap DNA + H2O Podospora anserina
-
 ?
-
 ?
additional information Podospora anserina recombinant protein is active on plasmid DNA, circular recessed and flap M13 substrate with short protruding single strand ?
-
 ?
ssDNA + H2O Podospora anserina
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Podospora anserina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Podospora anserina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsDNA + H2O
-
 Podospora anserina ?
-
 ?
flap DNA + H2O
-
 Podospora anserina ?
-
 ?
M13 flap DNA + H2O
-
 Podospora anserina ? small fragment of 5-10 nucleotides ?
M13 mp19 (+) DNA + H2O
-
 Podospora anserina ? small fragment of 5-8 nucleotides ?
additional information recombinant protein is active on plasmid DNA, circular recessed and flap M13 substrate with short protruding single strand Podospora anserina ?
-
 ?
additional information linear flap structures with tails of more than 20 nucleotides and shorter duplex regions are not hydrolyzed Podospora anserina ?
-
 ?
pUC19 DNA + H2O relaxation of the supercoiled DNA and cutting of the open circular DNA to a linear form Podospora anserina ?
-
 ?
ssDNA + H2O
-
 Podospora anserina ?
-
 ?
ssDNA + H2O
-
 Podospora anserina ? small fragment of 8-20 nucleotides ?

Subunits

Subunits Comment Organism
monomer 1 * 49000, SDS-PAGE, native mass by gel filtration Podospora anserina

Synonyms

Synonyms Comment Organism
NUC49
-
 Podospora anserina
rNUC49 recombinant NUC49 from Escherichia coli Podospora anserina