Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.30.2 extracted from

  • Oleson, A.E.; Janski, A.M.; Clark, E.T.
    An extracellular nuclease from suspension cultures of tobacco (1974), Biochim. Biophys. Acta, 366, 89-100.
    View publication on PubMed
Search for more literature for 3.1.30.2

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Nicotiana tabacum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for added divalent cation Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-nucleotides + H2O e.g. 3'-AMP Nicotiana tabacum ?
-
 ?
DNA + H2O native double-stranded Nicotiana tabacum 5'-phosphonucleotides + 5'-phosphomononucleotides
-
 ?
DNA + H2O single-stranded DNA Nicotiana tabacum 5'-phosphonucleotides + 5'-phosphomononucleotides
-
 ?
RNA + H2O rRNA Nicotiana tabacum 5'-phosphooligonucleotides + 5'-phosphomononucleotides
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
 native DNA Nicotiana tabacum
5.7
-
 denatured DNA Nicotiana tabacum
6
-
 RNA Nicotiana tabacum

pH Range

pH Minimum pH Maximum Comment Organism
4.2 9 adenosine 3'-monophosphate, less than 20% of maximal activity above and below Nicotiana tabacum
4.5 5.7 native DNA, less than 25% of maximal activity above and below Nicotiana tabacum
5 7 RNA less than 10% of maximal activity above and below Nicotiana tabacum