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Literature summary for 3.1.30.1 extracted from

  • Guan, Z.; Liu, J.; Bai, W.; Lv, Z.; Jiang, X.; Yang, S.; Chen, A.; Lv, G.
    Label-free and sensitive fluorescent detection of sequence-specific single-strand DNA based on S1 nuclease cleavage effects (2014), PLoS ONE, 9, e108401.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis development of a fluorescent method for simple detection of sequence-specific single-strand DNA in complex, contaminant-ridden samples directly without a DNA extraction and PCR step could simplify the detection of pathogens in the field and in the clinic. Upon clearing away redundant complementary ssDNA and possibly mismatched double strand DNA by using S1 nuclease, the fluorescent signal-to-noise ratio can be increased dramatically. It enables the method to be adaptable to three different types of DNA fluorescent dyes and the ability to detect target ssDNA in complex, multicomponent samples, like tissue homogenate from pork Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
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-
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Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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Aspergillus oryzae
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Synonyms

Synonyms Comment Organism
S1 nuclease
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Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Aspergillus oryzae