Cloned (Comment) | Organism |
---|---|
gene ENDO3, DNA and amino acid sequence determination and analysis, recombinant transient overexpression in Arabidosis thaliana leaf protoplasts | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
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Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme activity is not affected by Mg2+ or Fe2+ | Arabidopsis thaliana | |
Zn2+ | dependent on, activates at pH 5.5 | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | ENDO3 is the only nuclease, of all members of the Arabidopsis S1-like nuclease family, that demonstrates activity characteristic of fungal S1-type nucleases and mung bean plant nuclease, i.e. it digests ssDNA under acidic pH and in the presence of Zn2+ | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8LDW6 | gene ENDO3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | expression of ENDO3 is highly specific to flower development. It appears first in the shoot apex of an emerging inflorescence stem, remains high in young flowers and decreases gradually with the development of siliques. ENDO3 is preferentially expressed in the pistil and less abundantly in the stamen. The comparison of ENDO3 transcript levels in the whole pistil and separate ovules suggests that ENDO3 activity is mainly related to the ovule | Arabidopsis thaliana | - |
inflorescence stem | - |
Arabidopsis thaliana | - |
plant ovule | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
stamen | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ENDO3 is the only nuclease, of all members of the Arabidopsis S1-like nuclease family, that demonstrates activity characteristic of fungal S1-type nucleases and mung bean plant nuclease, i.e. it digests ssDNA under acidic pH and in the presence of Zn2+ | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ENDO3 | - |
Arabidopsis thaliana |
endonuclease 3 | - |
Arabidopsis thaliana |
nuclease I | - |
Arabidopsis thaliana |
plant S1-like nuclease | - |
Arabidopsis thaliana |
S1-like nuclease | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
optimal activity at acidic pH | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the plant S1-like nucleases class of enzymes. Different members of this family are characterized by a surprisingly large variety of catalytic properties, nucleolytic activities of all Arabidopsis thaliana S1-like paralogues, overview. In addition to Zn2+-dependent enzymes, this family also comprises nucleases activated by Ca2+ and Mn2+, which implies that the apparently well-known S1 nuclease active site in plant nucleases is able to cooperate with different activatory ions. Particular members of this class differ in their optimum pH value and substrate specificity. Plant representatives of this family evolve toward an increase in catalytic diversity. Phylogenetic analysis, overview. ENDO3 is the only nuclease, of all members of this family, that demonstrates activity characteristic of fungal S1-type nucleases and mung bean plant nuclease, i.e. it digests ssDNA under acidic pH and in the presence of Zn2+ | Arabidopsis thaliana |
physiological function | plant S1-like nucleases are the main class of enzymes involved in nucleic acid degradation during plant programmed cell death | Arabidopsis thaliana |