Literature summary for 3.1.30.1 extracted from

Liu, J.; Declais, A.C.; Lilley, D.M.J.
Mechanistic aspects of the DNA junction-resolving enzyme T7 endonuclease I (2006), Biochemistry, 45, 3934-3942.

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Protein Variants

Protein Variants	Comment	Organism
D55E	no cleavage of x strand 5'-labeled junction 3 in presence of Mg2+	Escherichia phage T7
E20D	limited cleavage of x strand 5'-labeled junction 3 in presence of Mg2+	Escherichia phage T7
E65D	no cleavage of x strand 5'-labeled junction 3 in presence of Mg2+, limited activity is restored in presence of Mn2+	Escherichia phage T7

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	mutant enzymes D55E and E65D show no cleavage of x strand 5'-labeled junction 3 in presence of Mg2+, mutant enzyme E20D shows limited cleavage	Escherichia phage T7
Mn2+	mutant enzyme E65D shoes limited cleavage of x strand 5'-labeled junction 3 in presence of Mn2+	Escherichia phage T7

Organism

Organism	UniProt	Comment	Textmining
Escherichia phage T7	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products	endonuclease I catalyzes the breakage of the P-O3' bond	Escherichia phage T7	a

Synonyms

Synonyms	Comment	Organism
T7 endonuclease I	-	Escherichia phage T7

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Release 2023.1 (January 2023)