Literature summary for 3.1.30.1 extracted from

Pimkin, M.; Miller, C.G.; Blakesley, L.; Oleykowski, C.A.; Kodali, N.S.; Yeung, A.T.
Characterization of a periplasmic S1-like nuclease coded by the Mesorhizobium loti symbiosis island (2006), Biochem. Biophys. Res. Commun., 343, 77-84.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Mesorhizobium loti

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	0.24 mM, complete inhibition of plasmid nicking activity	Mesorhizobium loti
MgCl2	50 mM, 25% inhibition	Mesorhizobium loti
NaCl	100 mM, 20% inhibition	Mesorhizobium loti

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasmic space	-	Mesorhizobium loti	42597	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
NaCl	maximal activity below 50 mM	Mesorhizobium loti

Organism

Organism	UniProt	Comment	Textmining
Mesorhizobium loti	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant carboxy-terminal hexahistidine tag fusion protein	Mesorhizobium loti

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	at acidic pH the enzyme is 25times more active on single-stranded DNA than on double-stranded DNA and 3times more active on single-stranded DNA than on single-stranded RNA. At neutral pH the RNase activity exceeds the DNase activity. The enzyme nicks supercoiled RF-I plasmid DNA and rapidly cuts the phosphodiester bond across from the nick in the resultant relaxed RF-II plasmid DNA.	Mesorhizobium loti	5'-phosphomononucleotides + 5'-phosphooligonucleotides	-	?

Synonyms

Synonyms	Comment	Organism
M1 nuclease	-	Mesorhizobium loti

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Release 2023.1 (January 2023)