Cloned (Comment) | Organism |
---|---|
cloned into a plasmid construct containing the T7 viral promoter, an N-terminal polyhistidine peptide, an Xpress epitope, and an EK cleavage site (pAtIMPH). Overexpression of soluble VTC4 protein in Escherichia coli strain pREP4/BL21(DE3) | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Arabidopsis thaliana | |
Li+ | - |
Arabidopsis thaliana | |
additional information | ascorbate is not an inhibitor of VTC4 | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.107 | - |
L-galactose 1-phosphate | pH and temperature not specified in the publication | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33100 | - |
x * 33100, calculated from sequence | Arabidopsis thaliana |
39700 | - |
x * 39700, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactose 1-phosphate + H2O | Arabidopsis thaliana | - |
L-galactose + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9M8S8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactose 1-phosphate + H2O | - |
Arabidopsis thaliana | L-galactose + phosphate | - |
? | |
L-galactose 1-phosphate + H2O | the bifunctional enzyme VTC4 also acts on myo-inositol phosphate (cf. EC 3.1.3.25) | Arabidopsis thaliana | L-galactose + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39700, SDS-PAGE | Arabidopsis thaliana |
? | x * 33100, calculated from sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
At3g02870 | - |
Arabidopsis thaliana |
VTC4 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | knockout lines of VTC4 exhibit small perturbations in abscisic acid, salt, and cold responses. Analysis of metabolite levels in vtc4 mutants show that less myo-inositol and ascorbate accumulate in these mutants | Arabidopsis thaliana |
physiological function | VTC4 is a bifunctional enzyme that impacts both myo-inositol and ascorbate synthesis pathways | Arabidopsis thaliana |