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Literature summary for 3.1.3.B9 extracted from

  • Torabinejad, J.; Donahue, J.L.; Gunesekera, B.N.; Allen-Daniels, M.J.; Gillaspy, G.E.
    VTC4 is a bifunctional enzyme that affects myoinositol and ascorbate biosynthesis in plants (2009), Plant Physiol., 150, 951-961.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloned into a plasmid construct containing the T7 viral promoter, an N-terminal polyhistidine peptide, an Xpress epitope, and an EK cleavage site (pAtIMPH). Overexpression of soluble VTC4 protein in Escherichia coli strain pREP4/BL21(DE3) Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Arabidopsis thaliana
Li+
-
Arabidopsis thaliana
additional information ascorbate is not an inhibitor of VTC4 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.107
-
L-galactose 1-phosphate pH and temperature not specified in the publication Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33100
-
x * 33100, calculated from sequence Arabidopsis thaliana
39700
-
x * 39700, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactose 1-phosphate + H2O Arabidopsis thaliana
-
L-galactose + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9M8S8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-galactose 1-phosphate + H2O
-
Arabidopsis thaliana L-galactose + phosphate
-
?
L-galactose 1-phosphate + H2O the bifunctional enzyme VTC4 also acts on myo-inositol phosphate (cf. EC 3.1.3.25) Arabidopsis thaliana L-galactose + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 39700, SDS-PAGE Arabidopsis thaliana
? x * 33100, calculated from sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
At3g02870
-
Arabidopsis thaliana
VTC4
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction knockout lines of VTC4 exhibit small perturbations in abscisic acid, salt, and cold responses. Analysis of metabolite levels in vtc4 mutants show that less myo-inositol and ascorbate accumulate in these mutants Arabidopsis thaliana
physiological function VTC4 is a bifunctional enzyme that impacts both myo-inositol and ascorbate synthesis pathways Arabidopsis thaliana