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Literature summary for 3.1.3.B8 extracted from

  • Cunha, S.; dAvo, A.F.; Mingote, A.; Lamosa, P.; da Costa, M.S.; Costa, J.
    Mannosylglucosylglycerate biosynthesis in the deep-branching phylum Planctomycetes: characterization of the uncommon enzymes from Rhodopirellula baltica (2013), Sci. Rep., 3, 2378.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.B8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rhodopirellula baltica
expression in Escherichia coli Rhodopirellula baltica

Inhibitors

Inhibitors Comment Organism Structure
EDTA 2 mM, no residual activity Rhodopirellula baltica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate pH 7.5, 25°C Rhodopirellula baltica
0.22
-
 2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate pH 7, 25°C Rhodopirellula baltica
6.16
-
 2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate pH 7.5, 40°C Rhodopirellula baltica
6.16
-
 2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate pH 7, 40°C Rhodopirellula baltica

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+ Rhodopirellula baltica
Mg2+ highest activity with 10 mM Mg2+ Rhodopirellula baltica
Mg2+ strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+. Maximum activity in presence of 10 mM Mg2+ Rhodopirellula baltica
Ni2+
-
 Rhodopirellula baltica
Ni2+ strictly dependent on divalent cations in decreasing order of efficiency: Mg2+, Ni2+, Co2+ Rhodopirellula baltica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35900
-
 x * 35900, calculated Rhodopirellula baltica
36000
-
 SDS-PAGE Rhodopirellula baltica

Organism

Organism UniProt Comment Textmining
Rhodopirellula baltica
-
-
-
Rhodopirellula baltica Q7UIS7
-
-
Rhodopirellula baltica DSM 10527 Q7UIS7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.013
-
 pH 7.5, 25°C Rhodopirellula baltica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O
-
 Rhodopirellula baltica 2-O-[2-O-(alpha-D-mannopranosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate
-
 ?
2-O-[2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O
-
 Rhodopirellula baltica DSM 10527 2-O-[2-O-(alpha-D-mannopranosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate
-
 ?
2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-3-phospho-D-glycerate + H2O
-
 Rhodopirellula baltica 2-O-[2-O-(alpha-D-mannopyrarosyl)-alpha-D-glucopyranosyl]-D-glycerate + phosphate
-
 ?
additional information enzyme is non-specific for the mannosyl-donor, using GDP-mannose, UDP-mannose and ADP-mannose as substrates with decreasing affinity. From the tested substrates only 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate is used as mannosyl-acceptor. The enzyme is a configuration retaining sugar transferase Rhodopirellula baltica ?
-
 ?
additional information enzyme is non-specific for the mannosyl-donor, using GDP-mannose, UDP-mannose and ADP-mannose as substrates with decreasing affinity. From the tested substrates only 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate is used as mannosyl-acceptor. The enzyme is a configuration retaining sugar transferase Rhodopirellula baltica DSM 10527 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35900, calculated Rhodopirellula baltica

Synonyms

Synonyms Comment Organism
MggB
-
 Rhodopirellula baltica
MGPG phosphatase
-
 Rhodopirellula baltica
RB12347
-
 Rhodopirellula baltica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Rhodopirellula baltica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15
-
 no activity below 15°C Rhodopirellula baltica
25
-
 28% of maximum activity Rhodopirellula baltica
90
-
 18% of maximum activity Rhodopirellula baltica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 half-live: 9.6 hours Rhodopirellula baltica
25
-
 half-life 9.6 h Rhodopirellula baltica
40
-
 half-live: 5.3 hours Rhodopirellula baltica
40
-
 half-life 5.3 h Rhodopirellula baltica
60
-
 28% activity retained Rhodopirellula baltica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Rhodopirellula baltica

pH Range

pH Minimum pH Maximum Comment Organism
4 9
-
 Rhodopirellula baltica

pI Value

Organism Comment pI Value Maximum pI Value
Rhodopirellula baltica calculated
-
 5.7