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Literature summary for 3.1.3.B4 extracted from

  • Cai, Y.; Deng, Y.; Horenkamp, F.; Reinisch, K.M.; Burd, C.G.
    Sac1-Vps74 structure reveals a mechanism to terminate phosphoinositide signaling in the Golgi apparatus (2014), J. Cell Biol., 206, 485-491.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.B4

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structure of yeast Sac1-HD (residues 2-449) is determined in a 1:1 complex with Vps74 (residues 64-341) Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C392S mutation leads to inactivation Saccharomyces cerevisiae
DELTA2-460 a truncated Sac1 mutant (Sac1(2-460), truncated at the end of the structured region) exhibits essentially no PtdIns4P phosphatase activity. In contrast to full-length version, presence of PtdIns4P in liposomes does not elicit substantial membrane binding by the truncated mutant Sac1(2-460), suggesting that residues 461-511 are important for substrate recognition Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Saccharomyces cerevisiae 5794
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae SacI is in complex with Vps74, a phosphatidylinositol 4-kinase effector ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information SacI is in complex with Vps74, a phosphatidylinositol 4-kinase effector Saccharomyces cerevisiae ?
-
 ?
phosphatidylinositol 4-phosphate + H2O preferred substrate Saccharomyces cerevisiae phosphatidylinositol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Sac1
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at room temperature Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
7.5
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction disruption of the Sac1-Vps74 interface results in a broader distribution of phosphatidylinositol 4-phosphate within the Golgi apparatus and failure to maintain residence of a medial Golgi mannosyltransferase Saccharomyces cerevisiae