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Literature summary for 3.1.3.B4 extracted from

  • Zhong, S.; Hsu, F.; Stefan, C.J.; Wu, X.; Patel, A.; Cosgrove, M.S.; Mao, Y.
    Allosteric activation of the phosphoinositide phosphatase Sac1 by anionic phospholipids (2012), Biochemistry, 51, 3170-3177.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylinositol allosteric activation Saccharomyces cerevisiae
phosphatidylserine allosteric activation Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C392S mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant shows no activity Saccharomyces cerevisiae
D394N mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant shows no activity Saccharomyces cerevisiae
D397N mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant shows no activity Saccharomyces cerevisiae
R207Q mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant is active but with reduced allosteric kinetics compared to that of the wild-type enzyme. Mutant shows a reduced allosteric effect of the R207Q mutation which is likely due to the reduced affinity for allosteric activators Saccharomyces cerevisiae
R256Q mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant shows no activity Saccharomyces cerevisiae
R398Q mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant shows no activity Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
monomer, ultracentrifugation Saccharomyces cerevisiae
96000
-
dimer, ultracentrifugation Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of Sac1 can be modulated by the lipid composition of the membrane bilayer Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Sac1 is active on PI(5)P, PI(4)P, PI(3)P, with low activity on PI(3,5)P2, and with no activity on other phosphoinositides Saccharomyces cerevisiae ?
-
?
phosphatidylinositol 4-phosphate + H2O preferred substrate Saccharomyces cerevisiae phosphatidylinositol + phosphate
-
?

Subunits

Subunits Comment Organism
dimer using ultracentrifugation enzyme exhibits two peaks with sedimentation coefficients of 3.1 S (55 kDa) and 4.3 S (96 kDa), corresponding to monomeric (80%) and dimeric (20%) forms of the protein, respectively Saccharomyces cerevisiae
monomer using ultracentrifugation enzyme exhibits two peaks with sedimentation coefficients of 3.1 S (55 kDa) and 4.3 S (96 kDa), corresponding to monomeric (80%) and dimeric (20%) forms of the protein, respectively. 95% of the protein is monomeric when the protein concentration is reduced to 0.1 mg/mL Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Sac1
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
? Sac1 shows a sigmoidal kinetic behavior. Sac1 undergoes an allosteric activation Saccharomyces cerevisiae