Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylinositol | allosteric activation | Saccharomyces cerevisiae | |
phosphatidylserine | allosteric activation | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C392S | mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant shows no activity | Saccharomyces cerevisiae |
D394N | mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant shows no activity | Saccharomyces cerevisiae |
D397N | mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant shows no activity | Saccharomyces cerevisiae |
R207Q | mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant is active but with reduced allosteric kinetics compared to that of the wild-type enzyme. Mutant shows a reduced allosteric effect of the R207Q mutation which is likely due to the reduced affinity for allosteric activators | Saccharomyces cerevisiae |
R256Q | mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant shows no activity | Saccharomyces cerevisiae |
R398Q | mutant is stable and displays a profile similar to that of the wild-type protein as determined by gel filtration chromatography. Mutant shows no activity | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
monomer, ultracentrifugation | Saccharomyces cerevisiae |
96000 | - |
dimer, ultracentrifugation | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of Sac1 can be modulated by the lipid composition of the membrane bilayer | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sac1 is active on PI(5)P, PI(4)P, PI(3)P, with low activity on PI(3,5)P2, and with no activity on other phosphoinositides | Saccharomyces cerevisiae | ? | - |
? | |
phosphatidylinositol 4-phosphate + H2O | preferred substrate | Saccharomyces cerevisiae | phosphatidylinositol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | using ultracentrifugation enzyme exhibits two peaks with sedimentation coefficients of 3.1 S (55 kDa) and 4.3 S (96 kDa), corresponding to monomeric (80%) and dimeric (20%) forms of the protein, respectively | Saccharomyces cerevisiae |
monomer | using ultracentrifugation enzyme exhibits two peaks with sedimentation coefficients of 3.1 S (55 kDa) and 4.3 S (96 kDa), corresponding to monomeric (80%) and dimeric (20%) forms of the protein, respectively. 95% of the protein is monomeric when the protein concentration is reduced to 0.1 mg/mL | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Sac1 | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
? | Sac1 shows a sigmoidal kinetic behavior. Sac1 undergoes an allosteric activation | Saccharomyces cerevisiae |