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Literature summary for 3.1.3.97 extracted from

  • Cummings, J.A.; Vetting, M.; Ghodge, S.V.; Xu, C.; Hillerich, B.; Seidel, R.D.; Almo, S.C.; Raushel, F.M.
    Prospecting for unannotated enzymes: discovery of a 3',5'-nucleotide bisphosphate phosphatase within the amidohydrolase superfamily (2014), Biochemistry, 53, 591-600.
    View publication on PubMed
Search for more literature for 3.1.3.97

Crystallization (Commentary)

Crystallization (Comment) Organism
unliganded enzyme, sitting drop vapor diffusion method, using 25% (w/v) PEG3350, 200 mM (NH4)2SO4, and 100 mM Bis-Tris (pH 6.5). Enzyme with AMP and phosphate bound in the active site, sitting drop vapor diffusion method, using 20% (w/v) PEG 2000 MME and 100 mM Bis-Tris (pH 6.0) Chromobacterium violaceum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
 5'-phosphoadenylyl-(3',5')-adenosine at pH 7.5 and 30°C Chromobacterium violaceum
0.0052
-
 2'-deoxy-guanosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
0.0054
-
 N6-methyl adenosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
0.0088
-
 uridine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
0.0093
-
 inosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
0.0102
-
 2'-deoxy-adenosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
0.014
-
 cytosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
0.0155
-
 adenosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
0.0173
-
 guanosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
0.022
-
 2'-deoxy-inosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
0.026
-
 2'-deoxy-cytosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
0.029
-
 2'-deoxy-uridine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM used in assay conditions Chromobacterium violaceum
Mn2+ the enzyme has three Mn2+ ions in the active site Chromobacterium violaceum

Organism

Organism UniProt Comment Textmining
Chromobacterium violaceum Q7NXD4
-
-
Chromobacterium violaceum ATCC 12472 Q7NXD4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxy-adenosine 3',5'-bisphosphate + H2O
-
 Chromobacterium violaceum 2'-deoxy-adenosine 5'-phosphate + phosphate
-
 ?
2'-deoxy-cytidine 3',5'-bisphosphate + H2O
-
 Chromobacterium violaceum 2'-deoxy-cytidine 5'-phosphate + phosphate
-
 ?
2'-deoxy-guanosine 3',5'-bisphosphate + H2O
-
 Chromobacterium violaceum 2'-deoxy-guanosine 5'-phosphate + phosphate
-
 ?
2'-deoxy-inosine 3',5'-bisphosphate + H2O
-
 Chromobacterium violaceum 2'-deoxy-inosine 5'-phosphate + phosphate
-
 ?
2'-deoxy-uridine 3',5'-bisphosphate + H2O
-
 Chromobacterium violaceum 2'-deoxy-uridine 5'-phosphate + phosphate
-
 ?
5'-phosphoadenylyl-(3',5')-adenosine + H2O lowest activity Chromobacterium violaceum ?
-
 ?
5'-phosphoguanylyl-(3',5')-adenosine + H2O
-
 Chromobacterium violaceum ?
-
 ?
adenosine 3',5'-bisphosphate + H2O second highest activity Chromobacterium violaceum adenosine 5'-phosphate + phosphate
-
 ?
cytidine 3',5'-bisphosphate + H2O
-
 Chromobacterium violaceum cytidine 5'-phosphate + phosphate
-
 ?
guanosine 3',5'-bisphosphate + H2O
-
 Chromobacterium violaceum guanosine 5'-phosphate + phosphate
-
 ?
inosine 3',5'-bisphosphate + H2O
-
 Chromobacterium violaceum inosine 5'-phosphate + phosphate
-
 ?
N6-methyl adenosine 3',5'-bisphosphate + H2O highest activity Chromobacterium violaceum N6-methyl adenosine 5'-phosphate + phosphate
-
 ?
uridine 3',5'-bisphosphate + H2O
-
 Chromobacterium violaceum uridine 5'-phosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer x-ray crystallography Chromobacterium violaceum

Synonyms

Synonyms Comment Organism
3',5'-nucleotide bisphosphate phosphatase
-
 Chromobacterium violaceum
Cv1693
-
 Chromobacterium violaceum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 5'-phosphoadenylyl-(3',5')-adenosine at pH 7.5 and 30°C Chromobacterium violaceum
3.3
-
 inosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
4.2
-
 2'-deoxy-guanosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
4.3
-
 2'-deoxy-inosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
5.2
-
 2'-deoxy-uridine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
5.4
-
 uridine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
7.1
-
 2'-deoxy-adenosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
8.6
-
 cytosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
9.4
-
 2'-deoxy-cytosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
11.6
-
 N6-methyl adenosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
12.3
-
 guanosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
22
-
 adenosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
32
-
 5'-phosphoadenylyl-(3',5')-adenosine at pH 7.5 and 30°C Chromobacterium violaceum
180
-
 2'-deoxy-uridine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
190
-
 2'-deoxy-inosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
360
-
 2'-deoxy-cytosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
360
-
 inosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
610
-
 uridine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
620
-
 cytosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
700
-
 2'-deoxy-adenosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
710
-
 guanosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
790
-
 2'-deoxy-guanosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
1400
-
 adenosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum
2100
-
 N6-methyl adenosine 3',5'-bisphosphate at pH 7.5 and 30°C Chromobacterium violaceum